Jul 8, 2000 · Catalytic DBU in the presence of an aliphatic or polymer-supported thiol rapidly removed the Fmoc group to provide the amine free base in excellent yields and purity on multi-gram scale.

In this study, alternative Fmoc-removal solution 2% DBU (1,8-diazabicyclo[5.4.0]undec-7-ene)/5% piperazine/NMP (N-methyl-2-pyrrolidone) led to drastic DKP reduction relative to 20% piperidine/DMF.

Here, we report an efficient Fmoc-deprotection solution containing piperazine and DBU which can cause complete removal of the Fmoc group in less than a minute. This combination rivals piperidine in speediness as revealed by kinetic studies.

May 1, 1991 · In this study, alternative Fmoc-removal solution 2% DBU (1,8-diazabicyclo[5.4.0]undec-7-ene)/5% piperazine/NMP (N-methyl-2-pyrrolidone) led to drastic DKP reduction relative to 20%...

Our results show that 5% piperazine + 2% DBU is faster than 20% piperidine and signi cantly reduces the occurrence of deletion sequences. arising due to incomplete Fmoc-deprotection in model sequence YA10K.

The deprotection step is crucial in order to secure a good quality product in Fmoc solid phase peptide synthesis. 9-Fluorenylmethoxycarbonyl (Fmoc) removal is achieved by a two-step mechanism reaction favored by the use of cyclic secondary amines; ...

The fluorenylmethoxycarbonyl protecting group (Fmoc) is a base-labile amine protecting group used in organic synthesis, particularly in peptide synthesis. [1] It is popular for its stability toward acids and hydrolysis and its selective removal by weak bases, such as piperidine , without affecting most other protecting groups or sensitive ...

In summary, we have reported a strategy of Fmoc removal with 2% DBU, 5% piperazine/NMP that could evidently minimize both the “traceless” and ordinary DKP formation in SPPS. This strategy could also enhance the Fmoc-removal kinetics compared …

Removal of Fmoc Protecting Group with DBU DBU-piperidine Deprotection Reagent. Dissolve 2.0 g of DBU and 2.0 mL of piperidine in DMF or NMP to make 100 mL. DBU Deprotection Procedure. Place the resin in a round bottom flask and add 20% (v/v) piperidine in DMF (approximately 10 mL/gm resin). Shake the mixture at room temperature for 2 minutes.

1,8-Diazabicyclo[5.4.0]undec-7-ene (DBU) removes the Fmoc protecting group much faster than piperidine. When Fmoc deprotection during a peptide synthesis is slow or incomplete, replacing piperidine with DBU can improve the deprotction yield …