The cell-permeant dye fluo-3 AM is a visible lightexcitable, high affinity Ca2+ indicator. It is available in 1 mg amounts (F-1241), as a 1 mM solution (F-14218), in a high-throughtput …

Fluo-3 is a fluorescence indicator of intracellular calcium (Ca 2+), developed by Roger Y. Tsien. [1] It is used to measure Ca 2+ inside living cells in flow cytometry, and confocal laser …

Fluo-3 AM是最常用的检测细胞内钙离子浓度的荧光探针之一。 分子式为C 51 H 50 Cl 2 N 2 O 23，分子量为1129.85。 Fluo-3和Fura-2相比，其优点是：一方面可以被氩离子激光 (argon …

The Ca 2+ indicator fluo-3 (Figure 1) was developed by Tsien and colleagues for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since being …

Cell-permeable acetoxymethyl (AM) ester of the fluorescent calcium indicator Fluo-3. Commonly used in cell-based experiments to detect changes in intracellular calcium levels. Practically …

Fluo-3 AM is a fluorecent Ca 2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM …

Membrane-permeable version of FLUO 3. Unlike all other Ca 2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg 2+.

Cell permeable fluorescent indicator of intracellular Ca 2+; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca 2+; lower binding affinity allows measurement of …

To check for possible AM ester degradation, the following simple test may be performed in a fluorometer. Dilute a small aliquot of AM ester stock solution to a final concentration of about 1 …

Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry. …