Fluo-3 is a fluorescence indicator of intracellular calcium (Ca 2+), developed by Roger Y. Tsien. [1] It is used to measure Ca 2+ inside living cells in flow cytometry, and confocal laser …

The Ca 2+ indicator fluo-3 (Figure 1) was developed by Tsien and colleagues for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy.

The cell-permeant dye fluo-3 AM is a visible lightexcitable, high affinity Ca2+ indicator. It is available in 1 mg amounts (F-1241), as a 1 mM solution (F-14218), in a high-throughtput …

Fluo-3 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM …

Since being introduced in 1989,1 fluo-3 imaging has revealed the spatial dynamics of many elementary processes in Ca2+ signaling.2-4 Fluo-3 has also been extensively used for flow …

Fluo-3 is a calcium sensor that utilizes fluorescein as a fluorescent tag, with excitation and emission wavelengths near 505 nm and 530 nm, respectively. It has a 325 nM affinity for Ca2+ …

Fluo- 3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry.

Cell-permeable acetoxymethyl (AM) ester of the fluorescent calcium indicator Fluo-3. Commonly used in cell-based experiments to detect changes in intracellular calcium levels.

Apr 5, 2025 · Fluo-3 is essentially nonfluorescent unless bound to Ca2+ and exhibits a quantum yield at saturating Ca2+ of ~0.14 and a Kd for Ca2+ of 390 nM. Fluo-3, pentapotassium salt is …

Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy …