Cell-type-specific and projection-specific brain-wide ... - Nature
Nov 19, 2018 · The fDIO (FLPo-dependent), dDIO (DreO-dependent) and vDIO (vCre-dependent) cassettes were constructed based on the DIO cassette (Figs. 1a and 3d).
分子表达操控中 Cre、Flp、Dre 等重组酶系统如何工作_公司新闻_ …
May 8, 2021 · 答案便是借助 Cre-LoxP 或 Flp-FRT 等重组酶系统。 简单来说，这类重组酶系统是通过特异位点重组酶 (Site-specific recombinases, SSRs) 介导重组酶特异识别位点 (Recombination tar-get sites, RTs) 间的重组，来实现特异位点的基因敲除、基因插入、基因翻转和基因易位等操作。 由于该技术能够有效克服其他类型重组技术的非特异性或重组效率低等缺点，近年来已逐渐在功能基因研究领域占据了主导地位。 Cre（Cyclization Recombination …
Intersectional, anterograde transsynaptic targeting of neurons ...
Feb 21, 2022 · Here, we developed a method to genetically label such neurons with a single gene of interest in mice by combining the anterograde transsynaptic spread of adeno-associated virus serotype 1 (AAV1)...
How Does Cre, Flp, Dre Recombinase System Work?
In addition to the Cre-LoxP system, there are similar vCre-vLoxP, sCre-sLoxP systems, and the Flp-FRT/F5 system and Dre-Rox1/Rox2 system that have no cross-effects with the Cre-LoxP system. The corresponding expression schemes are respectively fDIO and dDIO systems.
Application of AAV1 for Anterograde Transsynaptic Circuit …
Here, we present a simple approach for accessing postsynaptic neurons using AAV1 constructs that express a particular recombinase protein, such as Cre or FlpO.
tTARGIT AAVs mediate the sensitive and flexible manipulation of ...
Mar 11, 2021 · The Driver (AAV-hSYN1-fDIO-tTA) utilizes Flp-dependent Double-Floxed Inverted Open reading frame (fDIO) cassette (Fenno et al., 2014) to Flp-dependently invert tTA, permitting its expression by a human synapsin I (hSYN1) promoter.
pAAV-EF1a-fDIO-Cre - Addgene
Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase.
Generation and validation of a D1 dopamine receptor Flpo knock …
Mar 1, 2025 · We validated the Drd1-Flpo line by confirming Flp expression and functionality specific to D1DR+ neurons with immunohistochemistry and in situ hybridization. The Drd1-Flpo line is a useful resource for studying subpopulations of D1DR+ …
Central control of opioid-induced mechanical hypersensitivity and ...
Oct 14, 2024 · Repetitive use of morphine (MF) and other opioids can trigger two major pain-related side effects: opioid-induced hypersensitivity (OIH) and analgesic tolerance, which can be subclassified as mechanical and thermal. The central mechanisms underlying mechanical OIH/tolerance remain unresolved.
In this technique, the genes encoding site-specific recombinases (such as Cre recombi-nase, flippase (FlpO) recombinase and vCre recombinase) that recognize specific DNA sequences and can induce...