Floxing - Wikipedia
In genetic engineering, floxing refers to the insertion of a DNA sequence (which is then said to be floxed) between two LoxP sequences, creating an artificial gene cassette which can then be …
Lox-Stop-Lox TOPO - Addgene
Plasmid Lox-Stop-Lox TOPO from Dr. Tyler Jacks's lab contains the insert loxP-STOP-loxP and is published in Genes Dev. 2001 Dec 15. 15 (24):3243-8. This plasmid is available through …
A method for the generation of conditional gene repair mutations …
We present a method for the generation of conditional gene repair mutations that employs a loxP -flanked (floxed) selectable marker and transcriptional/translational stop cassette (neostop) …
Conditional Gene Expression Vector | VectorBuilder
5 days ago · This regular plasmid gene expression vector system utilizes the LoxP-Stop-LoxP (LSL) cassette to achieve Cre-mediated conditional activation of gene expression in …
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0 - Addgene
Plasmid AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0 from Dr. David Anderson's lab contains the insert G-CaMP3 and is published in Nature. 2013 Jan 31;493 (7434):669-73. doi: …
Cre+/-,flox/flox,fl/fl,flox/+,flox/- - 知乎专栏
flox/-: 表示该基因座为杂合,一个等位基因带有flox位点,另一个为敲除状态。 flox/flox: 表示该基因座为双重flox,两个等位基因均带有flox位点。 Cre-lox系统 中,flox位点可以使表达Cre酶的组织或 …
Utilizing the LoxP-Stop-LoxP System to Control Transgenic ABC ...
The LoxP-Stop-LoxP (LSL) system is a method to control transgenic expression at a defined time point. Within this system, transgene expression should only be initiated in the presence of the …
STOP floxing around: Specificity and leakiness of inducible …
Mar 3, 2020 · Here, upon tamoxifen administration, the Cre recombinase mediates excision of a loxP-flanked STOP cassette preceding the reporter gene, which subsequently allows the …
pcDNA3.1_Floxed-STOP mCherry - Addgene
Plasmid pcDNA3.1_Floxed-STOP mCherry from Dr. Moritoshi Sato's lab contains the insert pcDNA3.1_Floxed-STOP mCherry and is published in Nat Chem Biol. 2016 Dec;12 (12):1059 …
一文搞懂Cre-loxP条件性基因编辑 - 哔哩哔哩
May 29, 2024 · 在敲入模式中，STOP序列（转录终止元件）阻止目的基因表达，Cre通过重组loxP位点将STOP序列切除后、目的基因得以顺利表达，即实现了基因敲入效果。