May 30, 2022 · We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be...

The single-cell RNA-sequencing (scRNA-seq) field has evolved tremendously since the first paper was published back in 2009. While the first methods analysed just a handful of ce...

We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consump …

Among the latest breakthroughs in scRNA-seq is FLASH-seq(5), a cutting-edge plate-based method designed to enhance both the sensitivity and efficiency of single-cell RNA sequencing. In this blog, we explore how FLASH-seq is poised to reshape the …

Jun 20, 2022 · Plate-based single-cell RNA-sequencing methods have low throughput and require significant hands-on time. To overcome these limitations, we developed FLASH-seq, a full-length scRNA-seq protocol which can be performed in ~4.5 hours, is simple to automate and can be miniaturised to decrease costs.

Mar 23, 2022 · Building upon the existing Smart-seq2/3 workflows, we developed FLASH-seq (FS), a new full-length scRNA-seq method capable of detecting a significantly higher number of genes than both previous versions, requiring limited hands-on time and with a …

Nat Methods 10:1096-1098, 2013), we developed FLASH-seq (FS), a new full-length scRNA-seq method capable of detecting a significantly higher number of genes than previous versions, requiring limited hands-on time and with a great potential for customization (Hahaut et al. Lightning Fast and Highly Sensitive Full-Length Single-cell sequencing ...

Here, the authors develop their FLASH-seq protocol using human peripheral blood mononuclear cells (hPBMCs) from healthy individuals and from cultured HEK293T cells (an established line of human embryonic kidney cells). They sorted single cells using a flow cytometry approach into 96 well or 384 well plates.

FS will be especially useful for characterizing gene expression at high resolution across multiple samples. We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3.

Dec 11, 2022 · FLASH-seq low-amplification (FS-LA) represents the fastest method, which generates sequencing-ready libraries in 4.5 h, without sacrificing performance. FLASH-seq with UMIs (FS-UMI) builds upon the same principle as Smart-seq3 and introduces UMIs for molecule counting and isoform reconstruction.