FITC (fluorescein isothiocyanate) is widely used to label proteins, antibodies, peptides, hormones, amine-modified oligonucleotides, and other amine-containing molecules with the green fluorescent dye fluorescein.

Emission spectra of two fluorophores commonly used in flow cytometry, FITC and PE are shown. Also shown is a graphical representation of two commonly used filters, 525/50 and 585/40, to detect these fluorophores.

Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.

Nov 1, 2024 · In this report we demonstrated that the 405-nm violet laser excitation could be used to discriminate GFP and YFP signals. Using the violet laser to distinguish GFP and YFP has several advantages. First, the standard setup of the flow cytometer could be used, obviating the need for exchanging filters and dichroic mirrors.

First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC).

Rockland Anti-GFP Polyclonal, Catalog # 600-402-215. Tested in Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (Flow), Immunoprecipitation (IP) and ELISA (ELISA) applications.

FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser a... see more. Browse 33 more GFP / FITC antibodies.

Aug 24, 2017 · 如果你不了解 GFP、FITC 和 PE，你可能发表错误的流式数据。 当我们学习荧光素的时候，学到的第一点知识就是荧光素的发射波长比他们的吸收波长要长。 需要特别指出的是斯托克位移（stokes shift），斯托克位移对荧光素发光过程中非辐射能量的转移进行了总结。 当荧光素分子吸收光子的时候，一些吸收的能量在分子震动和运动（或者其它）中丢失，因此荧光后释放的能量比分子吸收的能量要低。 因为波长和能量成反比，所以发射光的波长比吸收光的波长 …

绿色荧光蛋白 (GFP)，是一个由约238个氨基酸组成蛋白质，从蓝光到紫外线都能使其激发，发出绿色萤光，在细胞生物学分子生物学中，绿色萤光蛋白 (GFP)基因常用做报告基因。 绿色萤光蛋白基因可以克隆到脊椎动物 (例如:兔子）上进行表现，并拿来映证某种假设的实验方法。 通过 基因工程技术，绿色萤光蛋白 (GFP)基因转进不同物种的基因组，在后代中持续表达。 现在，绿色萤光蛋白 (GFP)基因已被导入并表达在许多物种，包括细菌，酵母和其他真菌，鱼（例如斑马鱼）， …

You may notice this in your flow cytomtetry data when measuring FITC in the presence of PE, especially if using wide filters (e.g. 525/50 BP instead of 525/30 BP).