Oct 17, 2023 · An efficient and competitive NADP +-utilizing FDH necessitates the availability and robustness of NADPH regeneration systems. Herein, we report the engineering of a new FDH from Candida dubliniensis (CdFDH), which showed no strict NAD + preference by a structure-guided rational/semi-rational design.

Jun 8, 2020 · Here, we harness the power of natural selection to engineer FDH variants to simultaneously optimize three properties: kinetic efficiency with NADP +, specificity toward NADP +, and affinity toward formate.

Jul 24, 2024 · Formate dehydrogenases (FDHs) catalyze the oxidation of formate to CO 2 while reducing NAD (P) + to NAD (P)H and are classified into two main classes: metal-dependent (Mo- or W-containing) and metal-independent FDHs.

Aug 17, 2023 · NAD(P)+-dependent formate dehydrogenase (EC 1.2.1.2, FDH) catalyzes the simplest reaction from chemical and biological points of view, oxidation of formate-ion to carbon dioxide coupled to NAD(P)+ reduction to yield NAD(P)H. Advances in the life sciences have shown that this reaction plays an extremely important role in a wide variety of organisms.

Formate is an ideal electron donor for cofactor regeneration due to its general availability, low reduction potential, and benign byproduct (CO2). However, formate dehydrogenases (FDHs) are usually speci c to NAD+, such that NADPH regeneration with fi formate is challenging.

Formate dehydrogenase (FDH), a ubiquitous enzyme in prokaryotes and eukaryotes, catalyzes the reversible oxidation of formate to carbon dioxide (CO 2). FDHs are highly diverse in metal contents, subunit composition, types of redox cofactors, and their physiological roles (Hartmann et …

Dec 23, 2020 · In this review, all studies about the native NADP +-dependent FDHs and also engineered equivalents that reconstructed with different protein engineering approaches for altering the coenzyme specificity are outlined.

Dec 14, 2023 · Most natural formate dehydrogenases (FDHs) exhibit NAD + specificity, making it imperative to explore the engineering of FDH cofactor specificity for NADPH regeneration systems. The endogenous FDH of Komagataella phaffii (K. phaffii), termed KphFDH, is a typical NAD + -specific FDH.

Dec 1, 2009 · With the aim to develop novel NADP + -dependent formate dehydrogenase enzymes, simultaneous site-saturation mutagenesis of residues Asp195 and Tyr196 of CboFDH coupled with screening resulted in two mutant enzymes, D195Q/Y196R and D195S/Y196P, which showed significant NADP + specificity.

Jun 7, 2010 · A novel NADP +-dependent formate dehydrogenase (FDH) from five species within the Burkholderia cepacia complex (BCC) is reported here. These FDHs showed dual coenzyme specificity but preferred NADP + over NAD +, a property which has not been reported before.