6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents.

Fluorescein amidites, abbreviated as FAM, are important synthetic equivalents of fluorescein dye used in oligonucleotide synthesis and molecular biology. FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the complementary nucleic acids or primers for polymerase chain reaction .

FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5' or 3' end of oligos.

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

FAM fluorescence refers to the fluorescent properties of a dye known as Fluorescein Amidite (FAM). This dye is widely used in molecular biology and biochemistry for various applications, including DNA sequencing, qPCR, and fluorescence microscopy.

Jun 18, 2020 · Choose dyes with appropriate qPCR dye spectrum with appropriate excitation wavelengths and little to no overlap in their emission spectra. Consider total fluorescence intensity as well. For example, FAM is a good dye choice to identify low-copy transcripts because it has high fluorescent signal intensity.

FAM, or 6-carboxyfluorescein, is a fluorescent dye commonly used in quantitative Polymerase Chain Reaction (qPCR) assays. It is a reporter molecule that allows for the detection and quantification of a specific DNA sequence in real-time PCR applications.

Oct 29, 2021 · FITC and FAM are both derivatives of fluorescein. FITC, known as Fluorescein 5(6)-isothiocyanate, is a mix of isomers, which has a isothiocyanate reactive group (-N=C=S) at one of two hydrogen atoms on the bottom ring of the structure. 6-FAM also known as 6-carboxyfluorescein is a single isomer.

Fluorescein Amidite (FAM) is a widely used fluorescent dye in the field of molecular biology and biochemistry. It is particularly popular in applications such as real-time PCR, sequencing, and fluorescence microscopy. FAM is known for its bright green fluorescence.

5-FAM (5-Carboxyfluorescein) is a green fluorescent reagent used for in situ labeling peptides, proteins and nucleotides. 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm [1]. 5-FAM contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.