To verify the protein expression from IVT mRNA in vitro, primary cultured fibroblast-like synoviocytes (FLS) and MH7A human synovial fibroblast cells were transfected with enhanced green fluorescent protein (EGFP) mRNA with or without m1ψ incorporation.

FLS were transfected with in vitro-transcribed mRNA (IVT mRNA) and harvested at 24 h after transfection. a Western blotting of EGFP. b Images of FLS captured using fluorescence microscopy. Scale...

The mechanism of fibroblast-like synoviocyte (FLS) transformation into an inflammatory phenotype in rheumatoid arthritis (RA) is not fully understood. FLS interactions with invading leukocytes, particularly T cells, are thought to be a critical component of this pathological process.

Aug 4, 2016 · In this study, rotational and translational diffusions of enhanced green fluorescent proteins (EGFPs) and EGFP tandem-oligomer were measured. We succeeded in the determination of molecular...

Apr 1, 2002 · Centrifugal concentration of viral supernatant by 10- to 100-fold significantly increased the efficiency of viral transduction, with 50% or more of FLS expressing EGFP in several independent experiments using FLS lines from separate donors.

Mar 23, 2022 · To verify the protein expression of IVT mRNA in vitro, primary cultured human fibroblast-like synoviocytes (FLS) were transfected with enhanced green fluorescent protein (EGFP) mRNA with or without m1ψ incorporation. EGFP was detected using western blotting and fluorescence microscopy.

Mar 23, 2022 · 36 fibroblast-like synoviocytes (FLS) were transfected with enhanced green fluorescent protein 37 (EGFP) mRNA with or without m1ψ incorporation. EGFP was detected using western blotting

In this study, we aimed to evaluate the eficiency of gene expression and immunogenicity of exogenous m1ψ-incorporated IVT mRNA using a human fibro-blast cell line and primary cultured fibroblast-like synoviocytes (FLS). We demonstrate that lipofec-tion of m1ψ-incorporated IVT mRNA is a potential in vitro gene transfer technique for FLS.

We are developing retroviral-mediated gene transfer to human fibroblast-like synovial cells (FLS) as one approach to characterizing genetic pathways involved in synoviocyte pathophysiology. Prior...

The bait is localized to FLS by the fused C terminus of NS and visualized by the inherent fluorescence of the fused EGFP (Fig. 2c, left panels, middle and bottom rows).