Unleashed Actin Assembly in Capping Protein-Deficient B16-F1 …
To allow the most accurate quantification of F-actin content in CP-deficient B16-F1 cells, we trypsinized adherent wild-type and mutant cells and fixed and stained them with fluorescent …
F- and G-Actin Concentrations in Lamellipodia of Moving Cells
Mar 11, 2009 · In this work we established a method to determine the F- and G-actin concentrations in the lamellipodium. Our measurements demonstrate a local concentration of …
Actin in Action: Imaging Approaches to Study Cytoskeleton …
In this review we provide an introduction to various imaging modalities used to study focal adhesions and the actin cytoskeleton, and using specific examples we highlight a number of …
A role for actin arcs in the leading edge advance of migrating cells
Using a combination of live-cell imaging and photoactivation approaches, we demonstrate that the actin network of the lamellipodium evolves spatio-temporally into the lamella.
FAK and paxillin dynamics at focal adhesions in the protrusions of ...
To validate the results obtained on live cells using exogenous fusion proteins of FAK and actin filaments, we applied laser scanning confocal microscopy to visualize the endogenous FAK …
Fluorescence lifetime imaging: Association of cortical actin with …
Sep 4, 2008 · Time-lapse fluorescence lifetime images of a migrating B16-F1 melanoma cell showing a shift in the fluorescence lifetime of GFP–β-actin due to photobleaching. Color scale …
Revealing force- and orientation-dependence of F-actin …
Feb 8, 2024 · Mechanical forces play a pivotal role in this process, but the molecular mechanisms are still unclear. Recently, optical tweezers have been applied to study the interaction between …
Fast Single-Particle Tracking of Membrane Proteins Combined
6 days ago · Membrane protein dynamics regulates cell functions by initiating downstream signaling cascades. The cell membrane is compartmentalized into nanodomains by actin …
Ablation of actin filaments in a cell with a femtosecond pulsed laser ...
Download scientific diagram | Ablation of actin filaments in a cell with a femtosecond pulsed laser. Fluorescence images of actin filaments before (a) and after (b) laser dissection...
F-actin organization and target constriction during primary …
Dec 12, 2022 · We show that long-tailed myosins 1e/f are critical for this organization. Deficiency of myo1e/f causes dramatic shifts in F-actin localization, reducing F-actin at the phagocytic …