
EYFP :: Fluorescent Protein Database
EYFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer with …
Introduction to Fluorescent Proteins - Leica Microsystems
Sep 11, 2023 · Compared to GFP, excitation and emission properties have been shifted to longer wavelengths with excitation and emission maxima at 514 nm and 527 nm (EYFP). One …
A Guide to Choosing Fluorescent Protein Combinations for Flow ...
We demonstrate the utility of five fluorescent proteins with optimal characteristics for multicolor experiments, three of which can be easily used simultaneously (GFP or Venus, mTagBFP2 …
Yellow fluorescent protein - Wikipedia
Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. [1] Its excitation peak is 513 nm and its emission …
Perfusion fixation preserves enhanced yellow fluorescent protein …
Jan 1, 2009 · Enhanced yellow fluorescent protein (EYFP) was generated by introducing the following four mutations in to GFP: T203Y, S65G, V68L, and S72A. Together these mutations …
GFP Derivatives: CFP and YFP - News-Medical.net
Jan 25, 2019 · EYFP, the enhanced version of YFP, is the most widely used fluorescent protein. However, the pH sensitivity is very high in acidic conditions, as it loses half of its fluorescence …
A simple and effective method for differentiating GFP and YFP …
Nov 1, 2024 · In this report we demonstrated that the 405-nm violet laser excitation could be used to discriminate GFP and YFP signals. Using the violet laser to distinguish GFP and YFP has …
Enhanced Yellow Fluorescent Protein (EYFP) Nuclear Localization
Recombinant plasmids have been constructed that contain a fusion protein consisting of the yellow-green variant (referred to as enhanced yellow fluorescent protein; EYFP) of the …
Yellow Fluorescent Protein (YFP) Excitation - Nikon’s …
The Nikon YFP HYQ combination for enhanced yellow fluorescent protein (sometimes referred to simply as YFP, or yellow-emitting GFP) is one member of a continuum of filter sets that allows …
Mechanical Unfolding Pathways of the Enhanced Yellow Fluorescent ...
The enhanced yellow fluorescent protein (EYFP) is a particularly useful variant of GFP. EYFP is structurally equivalent to GFP with five additional mutations (S65G, V68L, Q69K, S72A, and …