The effects of caMEK and TGF-beta1 on collagen promoter activity were additive. These results indicate that ERK-dependent R-Smad linker region phosphorylation enhances collagen I synthesis and imply positive cross talk between the ERK and Smad pathways in human mesangial cells.

TGF-β binding to a Serine/Threonine kinase receptor complex causes the recruitment and subsequent activation of transcription factors known as smad2 and smad3. These proteins subsequently translocate into the nucleus to negatively or positively regulate gene expression.

We demonstrated that knock‐down of ERK1 instead of ERK2 reduced p‐Smad3 levels in whole cell extracts and the nuclear accumulation of p‐Smad3, which may suggest crosstalk between ERK1 and Smad2/3 in the regulation of TGF‐β signalling.

To examine the interaction of specific isoforms of ERK and the Smad2/3 signalling pathway, chondrocytes were infected with LV expressing either ERK1 or ERK2 siRNA and stimulated with or without TGF-β1.

Jan 10, 2020 · We found that the activation of Erk 1/2 signaling by TGF-β1, in healthy SGEC treated with IL-17, is independent of canonical IL-17-dependent TGFβ1/Smad signaling, because blocking Smad2/3...

Overexpression of the signaling protein, Smad3 enhanced TGF-β-induced activation of ERK MAPK whereas inhibition of Smad3 with ansiRNA blocked ERK MAPK phosphorylation in response to TGF-β. These data suggest that Smad3 acts as a signaling intermediate between TGF-β and ERK MAPK.

Mar 5, 2004 · We propose that TGF-beta (1) differentially regulates PSC activation, proliferation, and TGF-beta (1) mRNA expression through Smad2-, Smad3-, and ERK-dependent pathways, respectively. Pancreatic stellate cells (PSCs) play a major role in promoting pancreatic fibrosis.

Aug 23, 2005 · Ser 207 is the best ERK site in Smad3. Its phosphorylation shows the highest EGF induction in Smad3. It is also a very sensitive site to EGF treatment, significantly responding to low concentrations of EGF. These three sites are also phosphorylated by …

Mar 2, 2004 · We propose that TGF-β 1 differentially regulates PSC activation, proliferation, and TGF-β 1 mRNA expression through Smad2-, Smad3-, and ERK-dependent pathways, respectively.

Jun 17, 2003 · Transforming growth factor β (TGF-β) stimulates renal cell fibrogenesis by a poorly understood mechanism. Previously, we suggested a synergy between TGF-β1 activated extracellular signal-regulated kinase (ERK) and Smad signaling in collagen production by human glomerular mesangial cells.