Oct 2, 2020 · We revealed reciprocal regulation of early growth response 1 (EGR1) and EZH2: EGR1 activates the expression of EZH2, and EZH2 represses EGR1 expression. Using CRISPR-mediated genome/epigenome...

In this study, we developed an approach to systematically identify the trans-acting factors regulating the EZH2 expression, and identified more than 20 such factors.

EZH2 overexpression was described to play a crucial role in breast cancer pathogenesis, where it represses early growth response 1 (EGR1) expression, resulting in the inhibition of...

We report novel molecular connections between EGR1 and Polycomb Group function: Polycomb associated histone H3 lysine27 trimethylation (H3K27me3) blocks chromatin access of EGR1. In addition, EGR1 ablation results in abnormal Ezh2 and Bmi1 expression.

EZH2 is involved in the cell cycle, DNA damage repair, cell differentiation, autophagy, apoptosis, and immunological modulation. The main function of EZH2 is to catalyze the methylation of H3 histone of H3K27Me3, which inhibits the transcription of target …

Jan 11, 2018 · Antibodies against human CDK5, CDK5RAP1, H3K27me3, EZH2 (1:1000 dilutions, Cell Signaling Technology), Egr-1, E-cadherin, vimentin, β-catenin (1:1000 dilutions, Abcam), and β-actin (1:4000 dilutions, Santa Cruz) were used as primary antibodies.

Nov 26, 2020 · We revealed reciprocal regulation of early growth response 1 (EGR1) and EZH2: EGR1 activates the expression of EZH2, and EZH2 represses EGR1 expression. Using CRISPR-mediated genome/epigenome editing, we demonstrated that EHZ2 represses EGR1 expression through a silencer downstream of the EGR1 gene.

Jul 7, 2017 · EZH2, a histone methyltransferase, catalyzes H3K27me3, and it has been shown to be dysregulated in various cancers. EZH1 is an alternative core subunit to EZH2 in PRC2 complexes. Increasing evidence indicates that EZH2 is highly expressed in cancer stem cells and mediates cancer stem cell expansion and maintenance.

The Egr-1-binding protein Nab2 is an endogenous inhibitor of Egr-1-mediated signaling, and abrogates the stimulation of fibrotic responses induced by transforming growth factor-ß (TGF-ß).

Oct 2, 2020 · We revealed reciprocal regulation of early growth response 1 (EGR1) and EZH2: EGR1 activates the expression of EZH2, and EZH2 represses EGR1 expression. Using CRISPR-mediated genome/epigenome...