Apr 1, 2023 · Our objective was to examine a lipid nanoparticle (LNP)-based mRNA construct as a viable strategy to target alveolar epithelial cells and fibroblasts in IPF. mRNA-containing …

Aug 10, 2024 · Through optimizing the LNP nanomedicine via a “LOOP” process, we identified an optimal formulation capable of enduring shear force damage during nebulization.

Mar 11, 2025 · In this protocol, we detail steps for formulating representative mRNA LNPs (0.5 d) and characterizing key parameters (1–6 d) such as size, polydispersity index, zeta potential, …

Here, we have developed a library of N-series lipid nanoparticles (LNPs) that could specifically regulate the protein composition of protein corona on the surface of LNPs, which allows …

Jul 13, 2023 · Human retinal explants expressed GFP in cells with apical and basal processes consistent with Müller glia and in perivascular cells consistent with macrophages. We …

Aim: Increased knowledge of biodistribution and pharmacokinetics of lipid nanoparticle (LNP)-encapsulated mRNA drug components may aid efficacy and safety evaluation. Methods: Mice …

Apr 1, 2024 · We describe LNP delivery as a sequence of stochastic transfer processes and review a mathematical model of subsequent protein translation from mRNA. We compile …

We show that the thermodynamic state of the lipid membrane of the nanoparticle is a key design principle for acoustically responsive fusogenic nanoparticles. We have optimized a cationic …

EGFP is an enhanced version of GFP (green fluorescent protein), originally isolated from Aequorea Victoria, and shows a bright green fluorescence with an emission peak of 509nm. …

In this study, we report on the in vivo optimization of mRNA-loaded LNPs for use in vaccines. The screening included a wide range of methods for controlling particle size in addition to the …