Jul 25, 2016 · The lysine K85 in EGFP was targeted for OABK introduction because it undergoes crucial electrostatic and hydrogen-bonding interactions with a nearby aspartate, serine and cysteine, all key ...

Nov 5, 2014 · An EGFP mutant with an amber codon at position K85 (pEGFP-K85TAG) was generated as a fusion construct with mCherry, to provide a second reporter for successful plasmid transfection and incorporation of 1 and 2.

Sep 9, 2017 · We now report a novel genetically encoded fluorescent probe (EGFP-K85AcK) that responds to sirtuins in living cells. The probe design exploits a lysyl residue in EGFP that is essential for chromophore maturation, and is also an efficient deacetylation substrate for sirtuins.

Aug 8, 2021 · TRAF6 induced polyubiquitination of AURKB on K85 and K87, protruding on the surface of AURKB to facilitate its activation. AURKB–TβRI complexes in patient's tumor tissue sections correlated with the malignancy of prostate cancer.

Here, we report the high resolution (1.35 Å) structure of EGFP crystallised in its untagged sequence form that reveals the combined impact of the F64L and S65T, that give rise to improved folding and spectral characteristics.

Nov 6, 2022 · EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

Furthermore, two-photon and single-photon optochemical control of EGFP maturation was demonstrated, enabling the use of different, potentially orthogonal excitation wavelengths (365, 405, and 760 nm) for the sequential activation of protein function in live cells.

Enhanced GFP (EGFP). The first reports on EGFP indicated that the protein presented a single form and fluorescence peak, in contrast to the two conformations observed in wtGFP.

Enhanced green fluorescent protein. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. BsrGI is typically used …

Enhanced green fluorescent protein (EGFP) was selected as an initial target protein in order to test if this activation strategy would allow triggering of protein function in cells, since the activity of EGFP can be measured in real time using live cell imaging. 37 The lysine K85 in EGFP was targeted for OABK introduction, since it undergoes ...