Specific cell surface labeling of GPCRs using split GFP
Feb 9, 2016 · Here we present a rapid, specific and versatile labeling scheme for GPCRs at living-cell membrane with the use of a split green fluorescent protein (GFP).
- Author: Wen-Xue Jiang, Xu Dong, Jing Jiang, Yu-Hong Yang, Ju Yang, Yun-Bi Lu, San-Hua Fang, Er-Qing Wei, Chu...
- Publish Year: 2016
Highly multiplexed bioactivity screening reveals human …
Jul 6, 2023 · Next, we quantified the activation of twenty-four GPCRs by their canonical ligands via EGFP expression or quantitative polymerase chain reaction (PCR) (Figures S1 B and S1C). Both methods confirmed that each GPCR …
Exploring the dynamics of regulation of G protein-coupled …
Apr 23, 1999 · One GPCR which is highly resistant to agonist-induced short-term desensitization is the gonadotrophin releasing hormone (GnRH) receptor. This GPCR is unique in that, at …
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Chimeric GPCRs mimic distinct signaling pathways and modulate …
Aug 15, 2022 · We confirmed successful incorporation of GPCR chimeras in EGFP-expressing HMC3 cells through VSV-G immunostaining under non-permeabilizing conditions …
An atlas of GPCRs in dopamine neurons: Identification …
Jul 23, 2024 · Here, we isolate DANs from Dat -eGFP mice to generate a GPCR atlas by unbiased qPCR array expression analysis of 377 GPCRs. Combined with data mining of scRNA-seq databases, we identify multiple receptors in DAN …
Using green fluorescent proteins to study G-protein-coupled …
May 1, 2000 · Tagging of GPCRs with the green fluorescent protein (GFP) has enabled the direct visualization of real-time trafficking of GPCRs in living cells. Such analyses have provided …
- Author: Lorena Kallal, Jeffrey L Benovic
- Publish Year: 2000
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Detection of Ligand-activated G Protein-coupled Receptor ...
The pEGFP-N1 vector is a widely used and commercialized plasmid vector encoding an enhanced green fluorescent protein (EGFP), which has been optimized from wild-type GFP for …
EGF receptor activation by GPCRs: an universal pathway reveals ...
Jan 15, 2011 · About one decade ago has been demonstrated that G protein-coupled receptors (GPCRs) are able to utilize the epidermal growth factor (EGF) receptor (EGFR) as signalling …
Using EGFP fusions to monitor the functional expression of GPCRs …
Jan 19, 2008 · In combining fluorescence measurements with ligand binding assays, the versatility of the EGFP C-terminally fused to the human mu opioid receptor (EGFP-hMOR) has …
Live Cell Imaging of G Protein-Coupled Receptors
Jan 1, 2012 · Here, we summarize recent progress in live cell imaging of GPCRs using a confocal laser scanning microscope. The heptahelical G protein-coupled receptors (GPCRs) form a …
