EGFP :: Fluorescent Protein Database
Nov 6, 2022 · EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with …
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similar: EGFP. Add to collection. Add to favorites 34. mEGFP is a basic …
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Change History for EGFP. 2018-09-29 17:42, user: talley. added State EGFP …
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In EGFP, the direction of the transition dipole moment is 14° from the line …
Fluorescence-Activated Cell Sorting of EGFP-Labeled Neural Crest …
Fluorescence-activated cell sorting (FACS) is a flow cytometric method commonly used to isolate discrete populations of cells from heterogeneous cell pools. The technique of cell sorting …
Dual Fluorescence Cytometry Assay to Assess Cellular Protein Levels
Here, we provide a protocol for a dual-fluorescence flowcytometry-based assay to determine protein levels. The protein of interest is genetically linked to enhanced GFP (eGFP) followed …
Global Protein Stability Profiling in Mammalian Cells
We developed a “comparative GPS profiling” strategy to identify proteins whose stabilities increased or decreased in response to stimuli. We used two pools of …
- Author: Hsueh-Chi Sherry Yen, Qikai Xu, Danny M. Chou, Zhenming Zhao, Stephen J. Elledge
- Publish Year: 2008
A homozygous genome‐edited Sept2‐EGFP fibroblast cell line
After 10 days, we harvested cells and sorted them by FACS (fluorescence‐activated cell sorting) to distinguish a population of cells expressing Sept2‐EGFP from wild type (wt) cells. We found …
FACS analysis of EGFP-expression in neurons infected …
FACS analysis of EGFP-expression in neurons infected with lentivirus vector. FACS analysis were taken at 2, 3, and 4 days after primary cerebral cortical neurons were infected by...
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Purification of high-quality RNA from a small number of …
Mar 20, 2019 · We aimed to optimize a workflow to extract sufficient amounts of high-quality RNA from a limited number of FACS sorted cells from Tg (fli1a:GFP) zebrafish embryos, which can …
- Author: Siebe Loontiens, Lisa Depestel, Suzanne Vanhauwaert, Givani Dewyn, Charlotte Gistelinck, Charlotte G...
- Estimated Reading Time: 11 mins
- Publish Year: 2019
Simple Autofluorescence-Restrictive Sorting of eGFP
May 24, 2022 · Here we report a simple fluorescence activated cell sorting (FACS)-based enrichment method for direct assessment of efficacy and potential off-target effects of gene therapy co-delivered with an eGFP reporter to …
FACS analysis of EGFP expression in original and …
In this review, we focused on the possible concatenation mechanisms and how they could be studied with genetic reporters tracking individual copies in concatemers. We also discuss various features...
Application of EGFP-EGF fusions to explore mechanism of
The cell-binding and internalization activity of EGFP-EGF were analyzed by fluorescence-activated cell sorting (FACS) and confocal microscopy. Results: EGFP-EGF protein was …
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