In the presence of ATP, EcoP15I functions as a site-specific DNA endonuclease whose cutting site is 25-27 bp downstream of its recognition site, whereas in the presence of S …

Jun 12, 2015 · We show that the EcoP15I motor domains are specifically adapted to bind double-stranded DNA and to facilitate DNA sliding via a novel ‘Pin’ domain.

Oct 19, 2004 · EcoP15I is a Type III DNA restriction and modification enzyme of Escherichia coli. We show that it contains two modification (Mod) subunits for sequence-specific methylation of …

Dec 23, 2011 · EcoP15I consists of methylation (Mod) and restriction (Res) subunits forming a multifunctional enzyme complex able to methylate or to cleave DNA. In this study, we …

Here, we have studied the behavior of EcoP15I, using a novel fast-scan atomic force microscope, which uses a miniatur-ized cantilever and scan stage to reduce the mechanical response time …

Apr 17, 2009 · This report describes how the catalytic activity of Type III restriction endonuclease EcoP15I is influenced by cofactors, ionic conditions, enzyme concentration and particular …

Nov 18, 2024 · Firstly, chromatin digestion is carried out using DNase I, resulting in a higher resolution compared to digestion with restriction enzymes. Secondly, the use of EcoP15I to …

Feb 9, 2007 · We took advantage of the EcoP15I-overexpressing vector pQEP15 and affinity chromatography to generate a quantity of EcoP15I high enough for comprehensive proteolytic …

Oct 19, 2004 · After Ni-NTA affinity chromatography and ion exchange chromatography on heparin sepharose, we obtained 5mg homogeneous EcoP15I per gram cell pellet within 1-2 …

Feb 11, 2022 · We show that EcoP15I reduces DNA twist upon binding to its target site and subsequently uses ATP-hydrolysis to further reduce DNA twist, consistent with ∼5 bp of …