HDX under mildly denaturing conditions: Mixed EX1/EX2 Kinetics • The EX2 component of HDX kinetics appear to reflect rare and short global unfolding events (very large apparent ∆G; …

HDX-MS is a rapidly growing in structural biology, and automation of commercial HDX systems, together with streamlining of data analysis, provides an opportunity to do high-throughput …

Jul 25, 2019 · EX1 kinetics occurs when interconversion between folded and unfolded states is slower than the timescale of the HDX experiments, giving rise to a bimodal distribution of m/z …

Hydrogen/Deuterium eXchange Mass Spectrometry (HDX-MS) is a rapidly evolving technique for analyzing structural features and dynamic properties of proteins. It may stand alone or serve …

These “EX1” dynamics give rise to bimodal distributions where the relative intensity of the high mass component increases with time, and where 45–48. Using met-myoglobin (Mb) as model …

Nov 1, 2006 · Here we describe a data interpretation method involving peak width analysis that makes characterization of EX1 kinetics simple and rapid. The theoretical basis for EX1 and …

Oct 17, 2022 · We report HDX-MS analysis of the SARS-CoV-2 spike protein ectodomain in its trimeric pre-fusion form, which has 22 predicted N-glycosylation sites per monomer, with and …

Nov 1, 2006 · Here we describe a data interpretation method involving peak width analysis that makes characterization of EX1 kinetics simple and rapid. The theoretical basis for EX1 and …

Aug 17, 2023 · Residues 361–389 visually exhibit EX1 exchange pattern as seen in Figure S3. However, overlapping peaks from other peptides prevent the software from accurate binomial …

Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) is a hugely versatile and powerful technique that is experiencing a surge in application following advances in both software and …