Try Salt-T4 or Hi-T4 DNA Ligases. GMP-grade reagent also available. Learn more. Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini …

Improved sensitivity, specificity, and ligation efficiency with the addition of our new evolved KAPA EvoT4 DNA ligase, in the kit’s ligation ReadyMix. Excellent sequencing performance to …

The DNA ligase from bacteriophage T4 is one of the most widely used enzymes in molecular biology. It has evolved to seal single-stranded nicks in double-stranded DNA, but not to join …

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase …

Oct 28, 2005 · A mRNA that encodes the T4 DNA ligase gene and spacer peptide gene downstream of T4 DNA ligase is hybridized with dsDNA, a substrate of T4 DNA ligase. This …

Dec 23, 2019 · The EvoT4™ DNA ligase was developed to improve the efficiency and speed of adapter ligation for challenging and low-input DNA samples, thereby improving NGS sensitivity …

Oct 28, 2005 · T4 DNA ligase enzyme displayed on a ribosome, through addition of a spacer peptide, is able to react with dsDNA in the complex. The complex expressing active ligase was …

Jun 10, 2013 · We have fused T4 DNA ligase to seven different DNA-binding proteins, including eukaryotic transcription factors, bacterial DNA repair proteins and archaeal DNA-binding …

DNA ligase repairs broken DNA by forming a phosphodiester bond between a nearby 5’ phosphate and 3’ OH of the nicked or cut DNA strand. In addition to duplex DNA, T4 DNA …

Jun 20, 2024 · Yeasen Versatile T4 DNA ligase shows the lowest adapter dimer formation compared to the commercial T4 DNA ligases including thermotolerant ligases analyzed by …