May 12, 2022 · By comparing engineered embryonic stem cell differentiation and reprogramming models with multi-omics techniques, we demonstrated that ETV2 was able to bind nucleosomal DNA and recruit BRG1.

Nov 19, 2024 · In the present study, we examined the ETV2-RIG1 cascade as regulators that govern ETV2-mediated reprogramming. Mouse embryonic fibroblasts (MEFs) harboring an inducible ETV2 expression...

Nov 12, 2020 · We found that Brg1 maintains and stabilizes the binding of Etv2 on the nucleosome, and Etv2 requires Brg1 to activate downstream genes during reprograming.

BRG1 is an essential co-factor for ETV2 to function as a pioneer factor as Brg1 knockdown and conditional knockout significantly affected the ability of ETV2 to remodel chromatin and drive endothelial lineage formation in both reprogrammed fibroblasts and differentiating mESCs, respectively (37).

ETV2 has been shown to recruit the ATP-dependent chromatin remodeler, BRG1 (SMARCA4), to sustain an open configuration and increase H3K27ac deposition near the downstream endothelial genes.

Nov 14, 2022 · We also determined that ETV2 executed a pioneering role by recruiting and directly interacting with the ATP-dependent chromatin remodeling enzyme BRG1 to remodel chromatin around endothelial genes to help maintain an open configuration, resulting in increased H3K27ac deposition.

Jul 1, 2023 · Using mouse embryonic fibroblasts (MEFs), they elucidated that, at the initial step, ETV2 binds and recruits BRG1, an ATPase component of the SWI/SNF chromatin remodeling complex, onto closed chromatin domains to open chromatin of endothelial genes, as a …

By comparing engineered embryonic stem cell differentiation and reprogramming models with multi-omics techniques, we demonstrated that ETV2 was able to bind nucleosomal DNA and recruit BRG1.

We determined that ETV2 executes a pioneering role by recruiting BRG1 to remodel chromatin around endothelial genes and to help maintain an open configuration, resulting in increased H3K27ac deposition.

both MEFs and EBs, the "late" and "sustained" ETV2 bindings recruits BRG1, promotes H3K27ac deposition and chromatin remodeling to activate downstream endothelial programs.