LNP delivery of EPO mRNA to stimulate endogenous red blood cell (RBC) production may provide an alternative treatment to multiple invasive fetal blood transfusions for select fetal anemias, including those of infectious etiology.

Sep 24, 2019 · Endosomal escape of LNP-mRNA is dependent on the molar ratio between ionizable lipids and mRNA nucleotides. Our results show that fractions of ionizable lipids and mRNA (1:1 molar ratio of hEPO...

Dec 10, 2024 · We created a dataset of >9,000 lipid nanoparticle activity measurements and used it to train a directed message-passing neural network for prediction of nucleic acid delivery with diverse lipid...

Apr 2, 2024 · EPO is the perfect molecule for evaluating mRNA delivery from LNPs. Researchers utilized LNPs (iLP171) filled with several quantities of modified hEPO-mRNA to transfect Huh7 cells and study the...

Sep 30, 2024 · In the present work, mRNA (erythropoietin (EPO)) encapsulated LNPs were prepared using a next generation state-of-the-art patented, Sprayed Multi Absorbed-droplet Reposing Technology (SMART)...

Mar 15, 2024 · LNPs formulated with erythropoietin (EPO), responsible for stimulating red blood cell production, mRNA (858 base pairs), and the C13-200 IL led to EPO translation at levels similar to the C12-200 LNP.

Single-Dose Administration of EPO LNPs: LNP formulations encapsulating hEPO mRNA were injected IV at 0.5 mg kg −1 to male SD rats (7–8 weeks old) to assess lipid clearance.

Apr 3, 2024 · EGFP mRNA was produced in-house by Genevant for encapsulation in LNP for in-process lipid stability, and LNP zeta potential testing contained 5moU modification.

Dec 1, 2020 · Ionizable lipid nanoparticle (iLP171) was developed to deliver codon-optimized mRNA. Robust protein expressions of luciferase and erythropoietin (EPO) were achieved both in vitro and in vivo. ILP171/mRNA exhibited excellent safety profiles in vivo.

Lipid nanoparticles (LNPs) for mRNA delivery have advanced significantly, but LNP-mediated DNA delivery still faces clinical challenges. This study compared various LNP formulations for delivering DNA-encoded biologics, assessing their expression efficacy and the protective immunity generated by LNP-encapsulated DNA in different models.