Nov 15, 2022 · EdU as an alkyne-tagged Raman probe for detection of DNA in EC of various origins e.g. in human microvascular endothelial cells (HMEC-1), human aortic endothelial cells (HAoEC) and human coronary artery endothelial cells (HCAEC).

Recently a new flow-cytometric method to label and detect nascent DNA using EdU has been developed and made commercially available by Life Technologies [8]. EdU, is a nucleoside analog of thymidine that is incorporated into DNA during S-phase just like BrdU and is not reactive in biological systems [9].

Enterochromaffin (EC) cells (also known as Kulchitsky cells) are a type of enteroendocrine cell, and neuroendocrine cell. They reside alongside the epithelium lining the lumen of the digestive tract and play a crucial role in gastrointestinal regulation, …

Dec 12, 2023 · Endothelial cell (EC) generation and turnover by self-proliferation contributes to vascular repair and regeneration. The ability to accurately measure the dynamics of EC generation would advance our understanding of cellular mechanisms of …

Enterochromaffin (EC) cells synthesize 95% of the body 5-HT and release 5-HT in response to mechanical or chemical stimulation. EC cell 5-HT has physiological effects on gut motility, secretion and visceral sensation.

DNA Cell Proliferation assay was used to determine the impact of miR-139-5p on the cell proliferation of EC109 cells. A 2 h pulse of EdU was found to label approximately one-third of the...

Beginning from vasculogenesis, the angioblast-derived blood islands are specified through precisely timed and spatially coordinated networks of signaling pathways not only between themselves but also among other types of cells, which is …

Jun 29, 2017 · Serotonergic enterochromaffin (EC) cells are proposed to fulfill this role by acting as chemosensors, but understanding how these rare and unique cell types transduce chemosensory information to the nervous system has been hampered by their paucity and inaccessibility to single-cell measurements.

Jun 22, 2018 · Unexpectedly, prospective isolation reveals that AECs secrete nearly all detectable EC-derived stem cell factors (SCF). Genetic deletion of Scf in AECs, but not SECs, significantly reduced functional HSCs.

The Click-iT® EdU HCS Assay is a superior alternative to traditional methods for detecting and quantitating newly synthesized DNA.