Carboxyl-to-amine crosslinking using the carbodiimide EDC and Sulfo-NHS. Addition of NHS or Sulfo-NHS to EDC reactions (bottom-most pathway) increases efficiency and enables molecule (1) to be activated for storage and later use.

NHS esters are commonly used for protein modification (e.g. an NHS ester of fluorescein is commercially available, and can be added to a protein to obtain a fluorescently labeled protein in a straightforward reaction and purification step). NHS can be used with EDC to immobilize enzymes for biosensor applications. [4]

EDC is often used in combination with N-hydroxysuccinimide (NHS) for the immobilisation of large biomolecules. Recent work has also used EDC to assess the structure state of uracil nucleobases in RNA. [2] [3]

May 20, 2022 · EDC is now commonly used with N-hydroxysuccinimide (NHS) or its water-soluble analog, Sulfo-NHS, to activate carboxyl functional groups to form amine-reactive intermediates that spontaneously reacts with primary amines to form amide bonds.

For higher coupling efficiency and more stable amine-reactive intermediates, EDC protocols include N-hydroxysuccinimide (NHS) or its water-soluble analog (Sulfo-NHS).

Thermo ScientificTM EDC is a carboxyl and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond and release of an isourea by-product (see the Additional Information Section).

Homogeneous and dense collagen fibre bundles have been successfully produced through a multi-strand, broadly continuous extrusion process. Cross-linking with an EDC/NHS solution resulted in a highly aligned fibrillar structure with banding similar to …

In this study, the effect of crosslinking conditions on the properties of collagen films using EDC and NHS was investigated. Studies included FTIR spectroscopy, AFM, swelling and degradation tests, mechanical testing and contact angle measurements.

In this chapter detailed methods and problem discussions will be given to assist in fast decision analysis to optimize immobilization and regeneration. Topics in focus are different coupling techniques for small and large molecules, streptavidin-biotin sandwich immobilization, and optimizing regeneration conditions.

Apr 1, 2016 · In this paper, a novel pH-sensitive poly (amino acid) hydrogel based on poly γ-glutamic acid (γ-PGA) and ε-polylysine (ε-PL) was prepared by carbodiimide (EDC) and N-hydroxysuccinimide (NHS) mediated polymerization. The influence of PGA/PL molar ratio and EDC/NHS concentration on the structure and properties was studied.