Feb 1, 2021 · The ecdysone-inducible early gene E74 (Eip74EF) encodes an ETS (erythroblast transformation specific) transcription factor that mediates the 20E response in gene regulation.

In general, Br-C, E74, E75, and E93 positively affect 20E signaling (8). As a potent HTH transcription factor, E93 binds several 20E response genes and PCD genes on polytene chromosomes in Drosophila (16).

While indirect repression involves E74, EcR binds to an ecdysone response element different from those utilized in 20E-mediated gene activation to exert direct repressive action. Moreover, liganded EcR recruits a corepressor Mi2, initiating chromatin compaction.

We report here that a high level of Vg expression requires synergistic action of the EcR/USP and AaE74B through their direct protein-protein interaction on the Vg promoter. This study reveals how the 20E direct-indirect regulation of an effector gene is achieved at the molecular level.

Jun 26, 2023 · We found that ECR gene expression peaked in the 7-day-old larvae, then decreased gradually from the pupae stage. 20E slowly reduced food consumption and then induced starvation, resulting in small-sized adults. In addition, 20E induced ECR expression to regulate larval development time.

The 20E/EcR/USP complex binds to ecdysone response elements (EcREs) to activate early 20E‐response genes, for instance, Broad‐Complex (BrC), Ecdysone‐induced protein 75 (E75) and E74.

Oct 1, 2022 · E74 is a key transcription factor induced by 20E, which plays a broad role in many physiological events during insect growth and development, including vitellogenesis, organ remodeling and new tissue formation, programmed cell death and metamorphosis.

We report here that a high level of Vg expression requires synergistic action of the EcR USP and AaE74B through their direct protein–protein interaction on the Vg promoter. This study reveals how the 20E direct–indirect regulation of an effector gene is …

Oct 17, 2005 · Here we examined transcriptional activation of the vitellogenin (Vg) gene in the yellow fever mosquito, Aedes aegypti, by EcR/USP and E74 in response to an elevation of 20E titers.

We identified and characterized two isoforms of the Ets transcription factor E74 gene in B. mori (BmE74). In ASGs of B. mori last instar larvae, the Bm74A mRNA level increased concomitantly with an increase in haemolymph ecdysteroid titre after gut purge.