E2F is a group of genes that encodes a family of transcription factors (TF) in higher eukaryotes. Three of them are activators: E2F1, 2 and E2F3a. Six others act as repressors: E2F3b, E2F4-8. All of them are involved in the cell cycle regulation and synthesis of DNA in mammalian cells.

Using tagged-E2F knockin mice, imaging, and deep-learning tools, we mapped the spatial and temporal expression of E2F activators, canonical repressors, and atypical repressors during embryonic and adult development.

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.

Dec 3, 2015 · Our results define the binding specificity of the E2F family of transcription factors, and reveal the mechanism by which E2F8 is capable of regulating the cell cycle by directly repressing target genes activated by the typical E2F/DP heterodimers.

The size and complexity of the E2F family of proteins reflect a complexity in function with individual E2Fs performing both distinct and overlapping roles in proliferation, apoptosis, and development (15-17).

The positions of molecular size markers and E2F-specific bands are as in (A). To determine whether E2F-1 could be overexpressed in cells other than Ψ-CRE, virus collected from the pooled Ψ-CRE cells was used to infect three established murine fibroblast lines: Balb/3T3 clone A31, C3H10T1/2, and 3T3 clone 4 cells.

Apr 15, 1993 · E2F is a mammalian transcription factor that appears to play an important role in cell cycle control. DNA affinity column-purified E2F from HeLa cells reproducibly exhibits multiple protein bands when analyzed by SDS/PAGE.

Feb 9, 2009 · Atypical E2F proteins represent an evolutionarily conserved branch of the E2F TFs family, in which the presence of a duplicated DBD provides these new E2F players with the unique ability to bind DNA in a DP-independent manner.

In general, E2FA and E2FB are considered transcriptional activators, while E2FC is generally considered a repressor of cell proliferation (Sablowski and Gutierrez, 2022). In addition, it is well documented that E2FB at least, and probably E2FA, can also stimulate the G2/M transition.

Aug 1, 2004 · The E2F transcription factors are crucial for regulating cell cycle progression and tumorigenesis. Therefore, to understand these biological processes, we need to unravel the mechanisms by which these factors exert control.