Feb 20, 2020 · Using phage Mu transposition to measure the chromosomal conformation in live E. coli under physiological conditions reveals a well-mixed E. coli genome with no hard regional boundaries, but with some specific robust contacts among co-regulated gene groups.

The Escherichia coli chromosome or nucleoid is composed of the genomic DNA, RNA, and protein. The nucleoid forms by condensation and functional arrangement of a single chromosomal DNA with the help of chromosomal architectural proteins and RNA molecules as …

The Escherichia coli chromosome is a circular DNA molecule that is approximately 1000 times compacted in the living cell, where it occupies approximately 15% of the cellular volume. The genome is organized in a way that facilitates chromosome maintenance and processing.

Feb 8, 2018 · Here, we investigate the higher-order architecture of the Escherichia coli genome, showing its partition into two structurally distinct entities by a complex and intertwined network of contacts: the replication terminus (ter) region and the rest of the chromosome.

Jul 17, 2024 · In this study, we have shown that E. coli chromosome loci generally exhibit larger short-time-scale movement than the replisomes. Replication takes place within a restricted region into which...

Chromosomes isolated from E. coli cells were found early on to be folded by unconstrained negative supercoiling (Worcel and Burgi 1972). Unlike plasmids, which are relaxed by a single nick, a number of nicks were required to relax the chromosome completely.

May 1, 2008 · Like most characterized eubacterial chromosomes, E. coli has a single circular chromosome that is replicated bidirectionally from a unique origin, oriC, to create two chromosome arms or replichores.

May 12, 2004 · Jeong et al. analyzed similarities in the transcriptional activities of E. coli genes as a function of their position on the chromosome. An autocorrelation function identified three levels of spatial correlations of expressed genes: short-range (7-16 kilobase-pairs, kb), medium-range (approximately 100 kb) and long-range (over 700 kb).

Jun 12, 2024 · In this review, we will explore the history and recent advances that pave the way for a better understanding of E. coli chromosome organization and structure, discussing the cellular processes that drive changes in DNA shape, and what contributes to compaction and formation of dynamic structures, and in turn how bacterial chromatin affects key ...

Jul 8, 2019 · Here, we experimentally show in Escherichia coli that spatial confinement plays a dominant role in determining both the chromosome size and position. In non-dividing cells with lengths increased to 10 times normal, single chromosomes are …