As a potential mechanism underlying this discovery, our data suggest that 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC) is enriched on the surface of mRNA/LNPs with short-tail lipids. This feature limits their interaction with biological components, avoiding their …

We selected four such lipids: DMPC, DPPC, DSPC, and an HSPC (l -α-phosphatidylcholine, mixture of 11.4% DPPC and 88.6% DSPC) mixture. The measuring temperature in the experiments and simulations was selected at 295 K (22 °C).

The DSPC lipid had a cylindrical geometric structure and a small-sized head group originally, that forms the bilayer structure. Meanwhile, the DPSM lipid had a conical geometric structure and has also a larger head group than other lipids, which allows the liposome to form a micelle structure.

In this study, we present a CG-MD model for the mixture of DLPC, DMPC, DPPC, and DSPC in aqueous solution for a more detailed understanding of lipid mixture behavior in the bilayer state.

Dec 22, 2016 · The structural properties of two-component gel-phase bilayers of distearylphosphatidylcholine (DSPC) and alcohol molecules with different compositions and chain lengths (12-24 carbons long) are studied via molecular dynamics simulations.

Jan 1, 2006 · In this work, we report an atomic force microscopy (AFM) and fluorescence microscopy study of the distribution of gel-phase domains in supported lipid bilayers. Unlike most previous studies, the distribution of gel-phase lipid ranges from completely symmetric to completely asymmetric.

Sep 1, 2022 · Two distinct phases (as visualized by cryo-TEM), including a proposed oil-like phase containing the uncharged ionizable lipids and an aqueous phase, are pronounced for LNPs containing 20 mol% of the ionizable lipid KC2, 31.5 mol% DSPC, 47 mol% cholesterol, and 1.5 mol% PEG-lipid (Fig. 6D).

Dec 10, 2004 · Vesicles composed of long saturated diacyl chain phospholipids, namely DSPC and DPPC, were the most resistant to solubilisation, while those prepared from the shorter acyl chained DMPC were more readily solubilised.

Oct 18, 2001 · The PC lipids and the PEG:2000-PE polymer–lipids have the same acyl chain length of either dimiristoyl (DM) or distearoyl (DS) chains. DMPC/PEG:2000-DMPE and DSPC/PEG:2000-DSPE mixtures were investigated over the entire range of …

In this study the three lipids DSPC, DMPC, DPPC, all neutral, saturated lipids with different acyl chain lengths and phase Tc, were chosen to obtain optimum release kinetics in phosphate buffered saline (PBS) at 4 C (for storage conditions) and 37 C (to mimic mild biological con-ditions) over a 48-hr time period.