Mar 1, 2023 · Helper lipids, such as 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE) and 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC), are important components of LNP …

The researchers found superior potency of DOPE over DSPC and POPE for mRNA delivery. Similarly, Cheng Q et al. (Cheng et al., 2018) evaluated mRNA delivery in vivo using DOPE …

Jan 29, 2024 · The helper phospholipid (e.g. DSPC, DOPE, DOPC, etc.) increases LNP bilayer stability, which helps prevent leaking of nucleic acid cargo.

The results from the analysis of MD trajectories revealed that DOPE lipid headgroups associated strongly with lipid tails and carbonyl oxygens of DLin-MC3-DMA, while for DOPC lipid …

Feb 17, 2025 · DSPC, a saturated lipid that forms a rigid membrane, contributes to the stability of LNPs. DOPE is known to be cone-shaped due to a cis -oriented unsaturated bulky …

Feb 22, 2022 · The DSPC-containing LNP (LNP 90) was found to increase delivery to the spleen for both siRNA (two-fold) and mRNA (five-fold). In terms of liver delivery, the DOPE-containing …

A) The molecular shape of standard helper lipids, including DSPC, DOPE, and cholesterol, used in LNPs. Current commercial LNPs use both DSPC and cholesterol. B) The three different lipid …

Aug 10, 2021 · DSPC has been used in the mRNA-1273 and BNT162b2 COVID-19 vaccines 17. DOPE is a phosphoethanolamine with two unsaturated tails, which has a melting temperature …

Oct 1, 2024 · In this study, we compared the impact of three helper lipids (DSPC, DOPC, or DOPE) used with two different ionisable lipids (MC3 and C12–200) on stability, transfection …

Feb 4, 2021 · First, top-performing iPhos 9A1P9 exhibited 40- to 965-fold higher in vivo efficacy compared to previous benchmarks DOPE and DSPC.