Cre has long been recognized as the best SSR for genome engineering, particularly in mice. Obtaining another SSR that is as good as Cre will be a valuable addition to the genomic toolbox. To this end, we have developed and validated reagents for the Dre-rox system.

Rock your world with DRE-rox. A relative new-comer on the recombination scene is the D6 recombination system, or “Dre-rox.” Like Cre-lox, Dre-rox, also, is a bacteriophage-derived tyrosine recombinase in which the DreO recombinase recognizes a pair of identical “rox” sites and leaves behind a single rox site after

Nov 13, 2017 · The Dre–rox system permits rigorous control of Cre–loxP recombination in lineage tracing, effectively circumventing potential uncertainty of the cell-type specificity of Cre expression.

Jun 3, 2021 · The use of the dual recombinase-mediated intersectional genetic approach involving Cre-loxP and Dre-rox has significantly enhanced the precision of in vivo lineage tracing, as well as gene manipulation.

An explanation of using Dre/Rox technologies in the mouse for genetic lineage tracing.

Jun 3, 2021 · In this design, Dre-rox recombination switches Prox1-RSR-CreER into a Prox1-2A-CreER genotype. Tam-induced Cre-loxP recombination would subsequently result in genetic targeting in all organs and tissues of LECs only ( Figure 6 B).

The Dre/rox system is a second, more recently described alternative to the Cre/lox approach. As with Cre/lox, the Dre/rox system consists of a recombinase (DreO) that recognizes and recombines two identical DNA sequences (called rox sites), deleting any …

To enhance the precision of cell lineage tracing and functional study, a similar site-specific recombinase system termed Dre-rox has been recently used in combination with Cre-loxP.

Sep 2, 2009 · The Dre-rox system described by Sauer and McDermott (Sauer and McDermott, 2004) is important because it could be as remarkable as the Cre-loxP system, which is the best SSR that has been developed, so far, for genome engineering.

Dec 29, 2015 · We describe several rox variant sites that are incompatible with roxP, but are able to efficiently recombine with themselves in either purified systems or bacterial and eukaryotic tissue culture systems.