
DR5 is a Suitable System for Studying the Auxin Response in the
Sep 1, 2020 · DR5 is an artificially synthesized and highly active auxin-response element that contains seven tandem direct repeats of 11 bp, including the auxin responsive factor gene-binding site (TGTCTC) (Ulmasov et al., 1997).
A novel sensor to map auxin response and distribution at high …
Jan 15, 2012 · The tir1 afb1 afb2 afb3 quadruple mutant, DR5::GFP line and TIR1/AFB1–AFB3 GUS translational fusions have been described 4,19,31. DR5::VENUS transgenic plants were generated by transforming a...
DR5 as a reporter system to study auxin response in Populus
Jan 3, 2013 · DR5 activity was highest in cortex, suggesting high levels of auxin concentration and/or sensitivity in this tissue. Our study shows that the DR5 reporter system is a sensitive and facile system for monitoring auxin responses and distribution at cellular resolution in poplar.
Auxin-mediated statolith production for root gravitropism
May 20, 2019 · We found that the dramatically reduced DR5::GFP intensity and starch granule accumulation observed after l-Kyn treatment could be restored by extra application of NAA (Figs 1e, S1e, S2a).
Plant immune system activation is necessary for efficient root ...
Oct 13, 2021 · Inoculation by this bacterial strain stimulated lateral root formation and DR5::GFP expression (Figures S6 A and S6B), as well as triggering the immune response in an FLS2-dependent manner (Figure S6 C).
A reporter for noninvasively monitoring gene expression and plant ...
Sep 19, 2020 · The DR5:RUBY and DR5:eGFP in rice roots showed similar patterns (Fig. 2g), but DR5:RUBY was easier to observe without any treatments or a change in light conditions.
DR5-GFP expression in vegetative and inflorescence stage
DR5- GFP expression does not significantly differ from the wild type (WT; A) in pin1 seedlings (B). C and D, Vegetative stage II (4 weeks after germination). While wild-type plants have clearly...
Arabidopsis DR5::GFP reporter lines were grown vertically for …
To confirm the effect of SA treatments on IAA levels, a reporter line of Arabidopsis thaliana "DR5::GFP" was used [55]. ... Encapsulation Reduces the Deleterious Effects of Salicylic Acid ...
Reporters for sensitive and quantitative measurement of auxin …
Feb 2, 2015 · Nine repeats of either TGTCTC (DR5) or TGTCGG (DR5v2) are positioned in reverse orientation upstream of a minimal promoter and either nuclear 3×EGFP (n3GFP) or nuclear tandem Tomato (ntdTomato).
Arabidopsis DR5::GFP reporter lines were grown vertical | Open-i
To evaluate the contribution of auxin responsiveness in the altered primary root formation in response to P and Mg, we used transgenic plants expressing a DR5::GFP construct, which is useful in studying auxin-regulated gene expression in Arabidopsis (Ulmasov et al., 1997).