Diaphorase may refer to: Cytochrome b5 reductase, an enzyme NADH dehydrogenase, an enzyme NADPH dehydrogenase, an enzyme

Diaphorase enzymes are a class of dehydrogenase enzymes that catalyze reactions involving the nicotinamide adenine dinucleotide cofactors. Two specific members of this enzyme class, denoted as NADH diaphorase and NADPH diaphorase, are responsible for dehydrogenating the reduced forms of NAD and NADP, respectively.

Jan 6, 1999 · The diaphorases are a ubiquitous class of flavin-bound enzymes that catalyze the reduction of various dyes which act as hydrogen acceptors from the reduced form of di- and tri- phosphopyridine nucleotides, i.e., NADH, NADPH. The first such enzyme to be purified was that from heart muscle (Straub 1939).

The meaning of DIAPHORASE is a flavoprotein enzyme capable of oxidizing the reduced form of NAD.

The term “diaphorase” is a generic term for any oxidoreductase enzyme that can utilize either NADH or NADPH to catalyze the reduction of dyes (chromogenic electron acceptors), 10 including commonly used cell-based viability reporters such as MTT and methylene blue.

DT-diaphorase, also referred to as NQO1 or NAD (P)H: quinone acceptor oxidoreductase, is a flavoprotein that catalyzes the two-electron reduction of quinones and quinonoid compounds to hydroquinones, using either NADH or NADPH as the electron donor.

diaphorase (1) Dihydrolipoamide dehydrogenase, EC 1.8.1.4 (dihydrolipoamide:NAD+ oxidoreductase). (2) A generic term for any mitochondrial flavoprotein with reductase activity.

Diaphorase is a type of dehydrogenase that catalyzes reactions involving nicotinamide adenine dinucleotide cofactors. Two specific members of this class of enzymes, called NADH diaphorase and NADPH diaphorase, are responsible for the dehydrogenation of reduced forms of NAD and NADP, respectively.

NAD (P)H:quinone oxidoreductase (DT-diaphorase) appears to be a 2-electron transfer flavoprotein, which catalyzes the conversion of quinones into hydroquinones. Upon photoreduction in the presence of dimethylformamide, the enzyme forms a red semiquinone.

Diaphorase from Clostridium kluyveri has been used: in lactate dehydrogenase assay in gill cells. [1] impregnated on the strip for formate dehydrogenase assay. [2] in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor. for semiquinone generation during benzoquinone reduction. [3] for evaluation of glucose uptake in cultured muscle cells. [4] in …