Dihydrouridine (abbreviated as D, [1] DHU, or UH2) is a pyrimidine nucleoside which is the result of adding two hydrogen atoms to a uridine, making it a fully saturated pyrimidine ring with no remaining double bonds. D is found in tRNA and rRNA molecules as a nucleoside; the corresponding nucleobase is 5,6-dihydrouracil.

Sep 23, 2021 · We investigate the mechanism of crosslinking and use quantitative nucleoside liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis and 5-FUrd-based crosslinking and immunoprecipitation...

The D arm is a feature in the tertiary structure of transfer RNA (tRNA). It is composed of the two D stems and the D loop. The D loop contains the base dihydrouridine (D), for which the arm is named. [1] . The D loop's main function is that of recognition.

The dihydrouridine (D, sometimes DHU) RNA modification is a modified pyrimidine nucleoside whose corresponding nucleobase is 5,6-dihydrouracil. D is synthesized from uridine (U) by hydrogenation [Citation 8] (Fig. 1A).

Mar 12, 2024 · We introduce dihydrouridine sequencing (D-seq) for transcriptome-wide mapping of D with single-nucleotide resoln. and use it to uncover novel classes of dihydrouridine-contg. RNA in yeast which include mRNA and small nucleolar RNA (snoRNA).

Dihydrouridine (D) is a modified transfer RNA post-transcriptional modification (PTM) that occurs abundantly in bacteria, eukaryotes, and archaea. The D modification assists in the stability and conformational flexibility of tRNA.

Dihydrouridine is structurally unique. The dihydrouridine (D, sometimes DHU) RNA modification is a modified pyrimidine nucleoside whose corresponding nucleo-base is 5,6-dihydrouracil. D is synthesized from uridine (U) by hydrogenation [8] (Fig. 1A). Reduction of the uridine C5-C6 bond.

Apr 1, 1998 · Dihydrouracil (DHU) is a DNA base damage product produced in significant amounts by ionizing radiation damage to cytosine under anoxic conditions. DHU represents a model for pyrimidine base damage (ring saturation products) of the type recognized and repaired by Escherichia coli endonuclease III and its homologs in other species.

We investigate the mechanism of crosslinking and use quantitative nucleoside liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and 5-FUrd-based crosslinking and immunoprecipitation (CLIP) sequencing to map DUS3L-dependent dihydrouridine (DHU) modifications across the transcriptome.

Apr 1, 1998 · Dihydrouracil (DHU) is a DNA base damage product produced in significant amounts by ionizing radiation damage to cytosine under anoxic conditions. DHU represents a model for pyrimidine base damage (ring saturation products) of the type recognized and repaired by Escherichia coli endonuclease III and …