Denaturing High Performance Liquid Chromatography (DHPLC) is a method of chromatography for the detection of base substitutions, small deletions or insertions in the DNA. [1] Due to its speed and high resolution, this method is particularly useful for finding polymorphisms in DNA.

Denaturing high-performance liquid chromatography (DHPLC) compares two or more chromosomes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chromatography supports under partial …

Denaturing high-performance liquid chromatography (dHPLC) methods are ideal for scanning genes for novel mutations and for analyzing large sample sizes at low cost. dHPLC can also be used for genotyping specific mutations or polymorphisms.

dHPLC is a recently developed specialized version of high-performance liquid chromatography (HPLC). Whereas HPLC is a standard piece of equipment used in many crime labs for trace and drug analysis, dHPLC is currently available in only a few labs. The advantage of dHPLC over HPLC is applications in DNA testing. Current dHPLC

With the benefit of novel polymer chemistries used for separation, the accuracy, sensitivity, and the throughput of DHPLC for DNA and RNA analysis have greatly improved. DHPLC has been adopted in many laboratories for the screening of mutations and …

DHPLC is a chromatographic technique for the separation and analysis of DNA fragments with different length and/or base composition. This technique can be applied for mutation detection in DNA fragments of 200–1000 bp in length with a high …

Denaturing high-performance liquid chromatography (DHPLC) is an accurate and efficient screening technique used for detecting DNA sequence changes by heteroduplex analysis. It can also be used for genotyping of single nucleotide polymorphisms (SNPs).

Denaturing high-performance liquid chromatography (DHPLC) compares two or more chromo- somes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chro-

May 23, 2008 · Denaturing high-performance liquid chromatography (DHPLC) is a relatively new analytical technique that has been used primarily in human clinical medicine to detect variant PCR-amplified genes and disease mutations in biological samples containing mixtures of homologous genes (26 - 28, 37, 39).

Dec 1, 2001 · Denaturing high pressure liquid chromatography (DHPLC) is a relatively new technique, which uses heteroduplex formation between wild-type and mutated DNA strands to identify mutations.