Here we examined the capacity of EETs and DHETs to activate peroxisome proliferator-activated receptor-alpha (PPARalpha). We find that among the EET and DHET regioisomers, 14,15-DHET is the most potent PPARalpha activator in a COS-7 cell expression system.

Epoxyeicosatrienoic acids (EETs) are vasodilating lipid mediators metabolized into dihydroxyeicosatrienoic acids (DHETs) by soluble epoxide hydrolase. We aimed to develop a LC-MS/MS method to quantify EETs and DHETs in human plasma and monitored their levels during vascular endothelial stimulation.

Oct 23, 2013 · EETs are hydrolyzed by soluble epoxide hydrolase (sEH) to the corresponding dihydroxyeicosatrienoic acids (DHETs); thus, it is expected that the inhibition of this enzyme enhances the beneficial cardiovascular properties of EETs [5].

The CYP2C and CYP2J epoxygenases convert arachidonic acid into epoxyeicosatrienoic acid (EET) regioisomers (primarily 11,12- and 14,15-EET), which are converted into less-active dihydroxyeicosatrienoic acids (DHET) by soluble epoxide hydrolase (sEH).

Arachidonic acid is converted to epoxyeicosatrienoic acid (EET) by cytochrome P450 (CYP) epoxygenase. EETs primary metabolic fate is conversion to dihydroxyeicosatrienoic acids (DHETs) by the soluble epoxide hydrolase (sEH) enzyme.

We constructed multivariable-adjusted linear regression models to compare 11,12-EET, 14,15-EET, 11,12-DHET, 14,15-DHET, total EET, total DHET, CYP epoxygenase function (EET+ DHET), and sEH metabolic function (EET/DHET ratio) according to NAFLD phenotype and histologic severity.

Jan 1, 2006 · Here we examined the capacity of EETs and DHETs to activate peroxisome proliferator-activated receptor-α (PPARα). We find that among the EET and DHET regioisomers, 14,15-DHET is the most potent PPARα activator in a COS-7 cell expression system.

Nov 19, 2020 · Both in vitro and in vivo research suggests that epoxyeicosatrienoic acid (EET) protects against NASH. Four biologically active EETs are generated from arachidonic acid by cytochrome P450 (CYP) epoxygenases, and they are further metabolized to dihydroxyeicosatrienoic acids (DHETs) by soluble epoxide hydrolase.

Epoxyeicosatrienoic acids (EETs) are metabolized by soluble epoxide hydrolase (sEH) to form dihydroxyeicosatrienoic acids (DHETs) and are putative endothelium-derived hyperpolarizing factors (EDHFs). EDHFs modulate microvascular tone; however, the chemical identity of EDHF in the human coronary microcirculation is not known.

Jan 1, 2004 · Soluble epoxide hydrolase (sEH) converts EETs to dihydroxyeicosatrienoic acids (DHETs), and inhibition of sEH is a potential approach for enhancing the biological activity of EETs. EETs also undergo chain-elongation and β-oxidation, and the accumulation of partial β-oxidation products increases when sEH is inhibited.