Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. [1] . They are also known as 2',3' because both the 2' and 3' positions on the ribose lack hydroxyl groups, and are abbreviated as ddNTPs (ddGTP, ddATP, ddTTP and ddCTP). [2]

dNTP or deoxyribonucleotides contain deoxyribose as the sugar molecule. The nitrogenous bases are either purines or pyrimidines that interact through hydrogen bonds to form base pairs and carry genetic information. The four dNTPs are dATP, dGTP, dGTP and dTTP.

Nov 23, 2024 · To each reaction is added only one of the four dideoxynucleotides (ddATP, ddGTP, ddCTP, or ddTTP). Following rounds of template DNA extension from the bound primer, the resulting DNA fragments are heat denatured and separated by size using gel electrophoresis.

In this study, we describe the details of the 2.3-Å resolution crystal structures of three additional closed ternary complexes of Klentaq1 bound to a primer/template DNA and a ddATP, a ddTTP, or a ddGTP.

In this report, we present the details of the 2.3-A resolution crystal structures of three additional ternary complexes of Klentaq1 bound to a primer/template DNA and a dideoxyguanosine 5'-triphosphate (ddGTP), a dideoxythymidine 5'-triphosphate (ddTTP), or a dideoxyadenosine 5'-triphosphate (ddATP).

Deoxyguanosine triphosphate[1] (dGTP) is a nucleoside triphosphate, and a nucleotide precursor used in cells for DNA synthesis. The substance is used in the polymerase chain reaction …

ddNTP Set, 5 mM solutions (ddATP, ddCTP, ddGTP, ddTTP). Easy-to-use, time-saving solutions for use in any application requiring ddNTPs of high purity, such as dideoxy sequencing and genotyping reactions. High purity: ? 98% triphosphate.

Since DNA polymerase beta plays a role in DNA repair, the present results predict possible appearance of cytotoxicity or clinical side effect (s) of 2',3'-dideoxyguanosine (ddG), known as a potent inhibitor of human immunodeficiency virus, when ddG is administered to the patients with acquired immune deficiency syndrome (AIDS) or AIDS-related co...

Now, enter ddGTP, the mischievous twin brother of dGTP. This sneaky devil looks almost identical, but with one crucial difference: it's missing a tiny hydroxyl group, like a superhero without their cape. This seemingly insignificant detail throws a wrench in the whole DNA replication process.

ddGTP (2′,3′-Dideoxyguanosine 5′-triphosphate) trisodium is one of 2',3'-dideoxyribonucleoside 5'-triphosphates (ddNTPs) that acts as chain-elongating inhibitor of DNA polymerase for DNA sequencing. ddGTP trisodium acts as an inhibitor or a substrate for DNA polymerase α.