The direct antiglobulin test (DAT), also known as the Coombs test, is a crucial tool in diagnosing immune-mediated red blood cell (RBC) destruction (autoimmune hemolytic anemia).

Feb 1, 2017 · Studies using DAT results from healthy blood donors have found that up to 0.1% of healthy people have a positive DAT finding without evidence of hemolysis. 33 Of these, about …

The direct Coombs test, also referred to as the direct antiglobulin test (DAT), is used to detect if antibodies or complement system factors have bound to RBCs surface antigens. [5] The DAT …

Direct Coombs test (sometimes called a direct antiglobulin test) looks at your red blood cells to see if there are antibodies attached to them. It’s often used to diagnose blood-related …

Sep 12, 2022 · DAT testing typically involves using a polyspecific reagent consisting of IgG and complement C3. Indirect antiglobulin testing is clinically useful for the detection of circulating …

Sep 11, 2024 · The DAT is a serologic test to detect red blood cells (RBCs) that are coated with complement and/or antibodies in-vivo (in the body). Among physicians caring for patients at …

Jun 25, 2020 · In a patient with mixed-type autoimmune hemolytic anemia, the direct antiglobulin test commonly detects both IgG and C3. Paroxysmal cold hemoglobinuria is caused by a …

Direct Coombs Test is used to demonstrate in-vivo coating (in-vivo sensitization) of red cells with antibodies or complement in particular, IgG and C3d. Washed red cells from a patient or donor …

Direct Antiglobulin Test (DAT) with Reflex to Anti C3 and Anti IgG - The DAT (Direct Coomb's test) is positive if red cells have been coated, in vivo, with immunoglobulin, complement, or both.

To accomplish these goals, this review will discuss the following: (1) the history of the DAT, (2) how the test is performed in the clinical laboratory, (3) clinical situations for its use, (4) its …