009669 B6.129P2- Gt(ROSA)26Sor tm1(DTA)Lky /J When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor …

With the aim of developing such a system, we generated a ROSA26-eGFP-DTA mouse line, which combines the use of enhanced green fluorescent protein (GFP), diphtheria toxin A …

Here we describe a new knock-in allele at the Gt (Rosa)26Sor locus that retains the best features of previously described DTA alleles—including a CAG promoter, attenuated mutant DTA …

May 20, 2005 · We tested the iDTR strain by crossing to the T cell– and B cell–specific CD4-Cre and CD19-Cre strains, respectively, and observed efficient ablation of T and B cells after …

We demonstrate its utility by Cre-dependent ablation of both a broad domain in the embryonic nervous system and a discrete population of cells in the fetal gonads. We conclude that this …

Jun 20, 2019 · DTA exerts its toxic activity through inhibition of eukaryotic translation elongation factor 2 (eEF2) via adenosine diphosphate (ADP)-ribosylation of a modified histidine residue, …

These ROSA26-eGFP-DTA mice are useful for Cre-inducible deletion of specific groups of cells. C57BL/6J-congenic ROSA26-eGFP-DTA mice are available as Stock No. 032087 .

However, upon Cre-mediated excision of the floxed region DTA expression is activated, resulting in the specific ablation of Cre-expressing cells. As an example of this approach, we ablated …

The means to specifically ablate cells inside of a living organism have recently been improved and facilitated by stable mouse lines, carrying conditional expression constructs for diphtheria toxin …

Tamoxifen诱导Cre-ERT2小鼠 使用指南 Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结 …