DNase I is a versatile enzyme that nonspecifically cleaves DNA to release 5'-phosphorylated di-, tri-, and oligonucleotide products (1). A powerful research tool for DNA manipulations, DNase I is used in a range of molecular biology applications.

Use Deoxyribonuclease (DNase) I Solution (1 mg/mL) for routine tissue dissociation, minimizing clumping of concentrated and/or cryopreserved cell suspensions following thawing or enzymatic tissue digestion, and to eliminate DNA contaminants .

When using Thermo ScientificTM B-PERTM Reagent (Product No. 78248) or other lysis buffer to extract proteins, it may be necessary to degrade the genomic and plasmid DNA with DNase I to facilitate downstream applications. Trace amounts of …

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg 2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random.

Discover DNase I, grade II, an essential enzyme for DNA research. Find out more about its uses and applications here.

DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating random fragments for dideoxy sequencing. NOTE: for removing DNA from RNA preparations, use Amplification Grade DNase I (Cat. No. 18068-015).

DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

Deoxyribonuclease (DNase, for short) refers to a group of glycoprotein endonucleases which are enzymes that catalyze the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.

Use Deoxyribonuclease I (DNase I) for routine tissue dissociation, to minimize cell clumping, and eliminate DNA contaminants from dissociation medium. This endonuclease consists of a single glycosylated polypeptide chain with two disulfide bonds.

Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful for reducing or preventing aggregation of concentrated and/or cryopreserved cell suspensions following thawing. The activity range is ≥ 2000 Kunitz units per mg of protein.