- DNase Hi-C is a method to detect and quantify pairwise interactions between chromosome regions across the entire genome. DNase Hi-C was designed to overcome the limitations associated with restriction enzyme-based Hi-C approaches.data.4dnucleome.org/experiment-types/dnase-hi-c/
A cookbook for DNase Hi-C - Epigenetics & Chromatin
Our initial attempts to digest cross-linked chromatin using DNAse I showed that this step was not easy to reproduce. The distribution of fragment lengths obtained after digestion varied from sample to sample (Fig. 2a). Moreover, even at high enzyme concentrations, when the median fragment length was … See more
- 1. 1. Always check digestion and ligation of the chromatin using gel electrophoresis. Representative r…
- 2. 2. Estimate the streptavidin pulldown efficiency and compare the pulldown yield with th… See more
Mapping 3D genome architecture through in situ …
Sep 29, 2016 · Our method, termed DNase Hi-C, eliminates the reliance on restriction enzymes associated with Hi-C by digesting fixed chromatin with the …
- Author: Vijay Ramani, Darren A Cusanovich, Ronald J Hause, Wenxiu Ma, Ruolan Qiu, Xinxian Deng, C Anthony Bl...
- Publish Year: 2016
Mapping 3D genome architecture through in situ DNase Hi-C
We recently demonstrated that the inactive murine X chromosome adopts a bipartite structure using a novel 3C protocol, termed in situ DNase Hi-C. Like traditional Hi-C protocols, in situ …
- Author: Vijay Ramani, Darren A Cusanovich, Ronald J Hause, Wenxiu Ma, Ruolan Qiu, Xinxian Deng, C Anthony Bl...
- Publish Year: 2016
- Studies of DNase Hic
DNase Hi-C – 4DN Data Portal - 4D Nucleome Web …
Mar 28, 2019 · DNase Hi-C is a method to detect and quantify pairwise interactions between chromosome regions across the entire genome. DNase Hi-C was designed to overcome the limitations associated with restriction enzyme …
Using DNase Hi-C techniques to map global and local three
Jun 1, 2018 · We recently developed DNase Hi-C for mapping 3D genome organization, which uses DNase I for chromatin fragmentation. DNase Hi-C overcomes RE-related limitations …
- Author: Wenxiu Ma, Ferhat Ay, Choli Lee, Gunhan Gulsoy, Xinxian Deng, Savannah Cook, Jennifer Hesson, Christ...
- Publish Year: 2018
Targeted DNase Hi-C - PMC
We recently developed a Hi-C variant, DNase Hi-C, for characterizing 3D genome organization. DNase Hi-C employs DNase I for chromatin fragmentation, aiming to overcome restriction …
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We recently developed DNase Hi-C for mapping 3D genome organization, which uses DNase I for chromatin fragmentation. DNase Hi-C overcomes RE-related limitations associated with …
A cookbook for DNase Hi-C - PubMed
Mar 20, 2021 · We propose a new robust protocol for the preparation of DNAse Hi-C libraries from cultured human cells and blood samples supplemented with experimental controls and …
In this study, we compare diferent DNAse Hi-C protocols and identify the critical steps that significantly afect the eficiency of the protocol. In particular, we show that the SDS quenching …
DNase Hi-C — pitch-perfect chromatin mapping? - Nature
Dec 18, 2014 · Among other experiments, the team validated their approach in two human cell lines by coupling DNase Hi-C with a targeted DNA sequence capture technology to map fine …
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