Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of performing a specific chemical reaction, often but not always catalytic. This is similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA). [1] .

Sep 15, 2021 · DNAzyme-based biosensors offer various advantages over traditional detection strategies. Enzyme-linked immunosorbent assays (ELISAs) are one of the most common procedures used for the detection of target analytes.

Deoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are obtained by combinatorial in vitro selection methods.

Mar 25, 2021 · Here we report a xeno-nucleic-acid-modified version of the classic DNAzyme 10–23 that achieves multiple-turnover activity under cellular conditions and resists nuclease digestion.

In this study we provide an overview of an emerging class of nucleic acid therapeutic, called DNAzymes. In particular, we review the use of chemical modifications and carrier molecules for the stabilization and/or delivery of DNAzymes in cell and animal models.

Mar 30, 2021 · Now, a promising approach for constructing DNAzymes that show high gene-silencing efficiency in mammalian cells has been developed. This approach incorporates chemical modifications into an...

DNAzymes are generated de novo by in vitro selection--a powerful and yet simple technique that has been routinely used to isolate extremely rare DNA or RNA sequences with a function of interest (e.g. ligand-binding or catalysis) from an extraordinarily large population of single-stranded DNA or RNA molecules.

Sep 4, 2019 · We will begin with a description of the in vitro selection method with which an RNA-cleaving DNAzyme can be derived. This will be followed by a discussion on a few well-studied RNA-cleaving DNAzymes, with a focus on their properties and their demonstrated applications.

Jun 17, 2024 · High-precision DNA-based biocatalysts, known as DNA enzymes or DNAzymes, are experiencing a revival in biotechnological, analytical, and therapeutical applications.

DNAzymes, also known as deoxyribozymes, are synthetic single-stranded DNA molecules able to catalyze chemical reactions.