Aug 7, 2017 · Sequencing the DNA of the MRSA superbug can accurately identify patients most at risk of death and could help medics develop new treatments as we move towards personalised medicine, say...

Apr 2, 2023 · DNA polymerase chain reaction (PCR) of MRSA is the most sensitive test and gold standard test if cultures are inconclusive. DNA PCR of MRSA from nares is a frequently employed diagnostic test to rule out MRSA colonization.

Apr 12, 2024 · There are many methods laboratorians can use to test for MRSA. Four phenotypic methods are recommended for the detection of MRSA. The oxacillin and cefoxitin breakpoints for Staphylococcus aureus (S. aureus) are different than those for other staphylococci.

MRSA is any strain of S. aureus that has developed (through natural selection) or acquired (through horizontal gene transfer) a multiple drug resistance to beta-lactam antibiotics.

A molecular assay for the simultaneous detection of a Staphylococcus aureus-specific gene and the mecA gene, responsible for the resistance to methicillin in staphylococci, was evaluated. The assay included an automated DNA extraction protocol ...

The test utilizes the real-time PCR method for the amplification of an MRSA-specific DNA sequence recovered from clinical samples and fluorogenic target-specific hybridization with a molecular beacon probe for the detection of the amplified MRSA DNA.

May 24, 2019 · There is growing evidence for the value of bacterial whole-genome sequencing in hospital outbreak investigations. Our aim was to develop methods that support efficient and accurate low-throughput clinical sequencing of methicillin-resistant Staphylococcus aureus (MRSA) isolates.

Identification of MRSA strains was performed by detection of mecA gene in all S. aureus strains using PCR assay. The results revealed that 45.1% (126/279) of Staphylococci isolates carried mecA gene. The PCR-amplified DNA products of this gene of five selected clinical isolates are shown in Figure 1.

Jan 9, 2025 · The polymerase chain reaction (PCR) technique amplifies specific DNA sequences, making it possible to detect even minute quantities of genetic material. In MRSA detection, PCR targets unique genetic markers associated with antibiotic resistance.

In the past few decades, a more dangerous form of staph has emerged. This form is known as methicillin-resistant Staphylococcus aureus and is usually referred to by the acronym MRSA. What sets MRSA apart is that it is resistant to an entire class of antibiotics called beta-lactams.