10X reaction buffer supplied with T4 DNA Ligase. A 1X concentration assures optimal enzyme activity.

2X reaction buffer supplied with T3, T7, Hi-T4, and Salt-T4 DNA Ligases. A 1X concentration, assures optimal enzyme activity.

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3 hydroxyl and 5 phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 m

The 10X buffer has been tested for absence of endo-, exodeoxyribonucleases, ribonucleases and functionally tested in DNA ligation. For Research Use Only. Not for use in diagnostic procedures. The Thermo Scientific 10X T4 DNA Ligase buffer is used with T4 DNA Ligase.

High-concentration T4 DNA Ligase, in combination with the 2x Rapid Ligation buffer, greatly stimulates the rate and efficiency of blunt-end ligation; therefore, long incubations (>10 minutes) are NOT recommended and can significantly reduce the …

Buffer supplied with Taq DNA Ligase (NEB #M0208). NAD+, a cofactor required for Taq DNA Ligase, is included in the buffer.

Highly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required. Ampligase™ Thermostable DNA Ligase catalyses NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are …

Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation.

Ampligase Enzyme & Buffer contain: Ampligase DNA Ligase and 10X Reaction Buffer (25 μL Buffer for each 50 U enzyme). One unit of Ampligase is equal to as many as 15 units of other thermostable DNA ligases. Please compare competitive unit definitions.

Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature. Catalyzes the ATP-dependent joining of ssRNA or ssDNA onto the 5´-phosphoryl termini of ssRNA or ssDNA. Labels the 5´ end of ssDNA, dsDNA and RNA molecules to use as probes, in sequencing or DNA-protein footprinting.