Imaging of DNA and Protein–DNA Complexes with Atomic Force Microscopy
This article reviews atomic force microscopy (AFM) studies of DNA structure and dynamics and protein–DNA complexes, including recent advances in the visualization of protein–DNA …
Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy
This article describes sample preparation techniques for AFM imaging of DNA and protein–DNA complexes. The approach is based on chemical functionalization of the mica surface with …
Base-pair resolution analysis of the effect of supercoiling on DNA ...
Feb 16, 2021 · Here, we combine high-resolution atomic force microscopy (AFM) with molecular dynamics (MD) simulations to reveal how supercoiling affects global and local DNA …
Imaging of nucleic acids with atomic force microscopy - PMC
Atomic force microscopy (AFM) is a key tool of nanotechnology with great importance in applications to DNA nanotechnology and to the recently emerging field of RNA …
DNA accessibility of chromatosomes quantified by automated …
Sep 4, 2019 · DNA compaction and accessibility in eukaryotes are governed by nucleosomes and orchestrated through interactions between DNA and DNA-binding proteins. Using QuantAFM, …
AFM for analysis of structure and dynamics of DNA and protein–DNA ...
Mar 1, 2009 · This paper describes protocols for studies of structure and dynamics of DNA and protein–DNA complexes with atomic force microscopy (AFM) utilizing the surface chemistry …
Atomic Force Microscopy of DNA and DNA-Protein Interactions
May 31, 2022 · Here, we describe the application of AFM to determine the secondary and tertiary structure of surface-bound DNA, and its interactions with proteins. Atomic force microscopy …
Imaging DNA Structure by Atomic Force Microscopy
Jun 10, 2016 · Here, we describe the application of AFM to determine superstructure and secondary structure of surface-bound DNA. The method is also readily applicable to probe …
Organisation and dynamics of individual DNA circles in Kinetoplast DNA
1 day ago · The kinetoplast DNA (kDNA) is a topologically complex genome, made by thousands of DNA (mini and maxi) circles interlinked into a two-dimensional Olympic network. ... using …
Nanotechnology meets medicine: applications of atomic force microscopy ...
Apr 3, 2025 · Atomic force microscopy (AFM) is a scanning imaging technique able to work at the nanoscale. It uses a cantilever with a tip to move across samples’ surface and a laser to …