
DiI-Labeling of DRG Neurons to Study Axonal Branching in a …
Here we present a technique to label the trajectories of small groups of DRG neurons into the embryonic spinal cord by diffusive staining using the lipophilic tracer 1,1'-dioctadecyl-3,3,3',3' …
Over 30 Years of DiI Use for Human Neuroanatomical Tract …
A combination of DiI tracing and immunohistochemistry in double-labeling studies have been shown to provide the means for assessing connectivity of phenotypically defined human CNS …
Histological Methods for ex vivo Axon Tracing: A Systematic Review
From analyzing Table 2, it is apparent that the most frequently used tracer is DiI. Twenty out of the 23 studies referenced in Table 2 use some form of DiI as the preferred axon tracer. This …
Sulfonated derivatives of DiI and DiO, and CM-DiI (a thiol-reactive DiI derivative) pro-duce staining that persists after fixation and permeabilization treatments.
Protocol for immunostaining of DiI-labeled cells
Immunostaining of singly DiI-labeled mouse cortical neural progenitor cells and their daughter cells. (following live observation of these cells in slice culture) by Takaki Miyata. January 17, …
Jun 28, 2016 · The simplest approach to obtain an alternative dye is to chemically change an existing dye, e.g. DiI, into a DiI—analogue, which possesses both the property of adhering to …
DiI-labeling of DRG neurons to study axonal branching in a ... - PubMed
Dec 13, 2011 · Here we present a technique to label the trajectories of small groups of DRG neurons into the embryonic spinal cord by diffusive staining using the lipophilic tracer 1,1' …
DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging
Gerhardt et al. develop the X-ray-visible neural tracer DiI-CT based on the well-established indocarbocyanine dye DiI. The authors demonstrate the dye’s potential by examining the rat …
Fluorescent labeling of dendritic spines in cell cultures with the ...
May 8, 2014 · Our protocol described here results in high quality staining and imaging of dissociated cell cultures with lipophilic DiI labeling and confocal microscopy. This visualization …
DiI-Labeling of DRG Neurons to Study Axonal Branching in a …
Dec 13, 2011 · Here we present a technique to label the trajectories of small groups of DRG neurons into the embryonic spinal cord by diffusive staining using the lipophilic tracer 1,1' …