DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [1] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.

DH5α is one of the most common laboratory E. coli strains utilized for small plasmid propagation and is selected for its ability to achieve high transformation efficiency.

In very simple way DH5 alpha was designed for higher stability and transformation by genetic manipulations (mutations) while BL 21 for higher level of protein expression.

NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of …

One of the most common laboratory E. coli strains used to maintain and amplify small plasmid DNA is K-12 derived DH5α. Typical DNA assembly and cloning procedures involve as their last step transformation of the constructed plasmid into competent DH5α cells.

Sep 13, 2019 · Transforming of recombinant DNA using DH5a E. coli competent cells. Refreeze unused cells on dry ice then into -80 °C. Add 1 μL of each new ligation (experiment tube) per …

We successfully transformed Escherichia coli DH5alpha prepared using the normal calcium chloride method, with cosmids up to 30 - 35 kb.

The new features of DH5 alpha are the recA and the endA1 mutations: The endA1 mutation inactivates an intracellular endonuclease that degrades plasmid DNA. The recA mutation eliminates homologous recombination. This reduces the chance for deletions and plasmid multimerization. The DH5 strain also has the following features:

Complete Genome Sequence of NEB 5-alpha, a Derivative of Escherichia coli K-12 DH5α. Genome Announc. 4 (6), e01245-16. PMID- 27834703; Full-Text Links: ASM (Download PDF), PMC. Song et al., 2015. Determination of single nucleotide variants in Escherichia coli DH5α by using short-read sequencing. FEMS Microbiol Lett. 362 (11), fnv073.

Description: DH5a™ is the most frequently used E. coli strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a™ increase insert stability and improve the quality of plasmid DNA prepared from minipreps. Application: Highest transformation efficiency General cloning Blue-white ...