Mar 5, 2015 · In order to follow the Ddx4 disordered regions within a cell, we generated a mimic, Ddx4 YFP, in which the DEAD-box helicase is substituted by YFP, a fluorescent protein of …

Dec 16, 2022 · On the other hand, as live-cell models, we use mouse fibroblasts expressing DDX4-YFP, which forms liquid-like nuage granules that are partitioned from chromatin 16, and …

Sep 9, 2017 · Ddx4 contains an intrinsically disordered N terminus of ∼ 250 residues, a DEAD-box RNA helicase, and a disordered C terminus of ∼ 50 residues. A construct of Ddx4 …

May 16, 2016 · The WT Ddx4 protein is a primary constituent of nuage membraneless organelles that occurs with a DEAD box helicase domain in place of the YFP moiety in Ddx4 YFP studied …

Sep 11, 2017 · We show that the protein within the concentrated phase of phase-separated Ddx4, Ddx 4 cond, diffuses as a particle of 600-nm hydrodynamic radius dissolved in water. …

Mar 5, 2015 · A Ddx4 construct containing both YFP and the DEAD- box helicase domain similarly formed organelles, although they were observed to form in the cytoplasm (Figure S2C).

Apr 7, 2023 · The Ddx4 YFP mutant, which has a yellow fluorescent protein (YFP) instead of the central RNA helicase domain, also assembled the droplets, albeit in the nucleus. The N-IDR …

Efficient buffering of 2NT-DDX4 YFP expression noise in HeLa cells through phase separation. (A) Time-lapse microscopy of a typical cell initiating expression of 2NT-DDX4 YFP following...

Jan 24, 2020 · To test the concept of noise reduction inside cells, we used a recombinant 2NT-DDX4 YFP protein that phase-separates in vitro (fig. S21). We expressed 2NT-DDX4 YFP …

In order to follow the Ddx4 disordered regions within a cell, we generated a mimic, Ddx4 YFP, in which the DEAD-box helicase is substituted by YFP, a fluorescent protein of similar …