The minisatellite marker D1S80 is located at 1p35-p36. This VNTR is 16 bases long. With a variation of alleles between 3-24 repeats, the locus displays enough diversity to aid in distinguishing …

The highly polymorphic minisatellite locus D1S80, (gene location: 1p36.32, GenBank sequence Accession # D28507) was first described by Nakamura in 1989. It is a minisatellite locus composed of short repeat units, typically less than or equal to 16 base pairs per unit [16, 17] (Table 1, Figure 1).

Compared with RFLP analysis of VNTR loci by Southern blotting, the approach described in this paper offers certain advantages: (1) discrete allele resolution, (2) minimal measurement error, (3) correct genotyping of single-band VNTR patterns, (4) a nonisotopic assay, (5) a permanent record of the electrophoretic separation, and (6) reduced assay...

Hypervariable loci within the human genome are useful tools in several disciplines: for example in forensic medicine (paternity testing and forensic identification). One of these genetical markers is located at chromosome 1 and is called D1S80 (MCT118).

Mar 1, 2004 · A sequenced allelic ladder marker that contains 32 alleles consisting of alleles 13–44 was developed for D1S80 (MCT118) typing. Each allele, after amplification with D1S80 primers, was cloned...

Compared with RFLP analysis of VNTR loci by Southern blotting, the approach described in this paper offers certain advantages: (1) discrete allele resolution, (2) minimal measurement error, (3) correct genotyping of single-band VNTR patterns, (4) a nonisotopic assay, (5) a permanent record of the electrophoretic separation, and (6) reduced assay...

The minisatellite marker D1S80 is located at 1p35-p36. This VNTR is 16 bases long. With a variation of alleles between 3-24 repeats, the locus displays enough diversity to aid in distinguishing between people. Although this is not a CoDIS marker, use of multiple loci are required to definitively identify samples.

These characteristics of the D1S80 locus make it a very useful marker for population genetic research, genetic linkage studies, forensic identification of individuals, and for determination of biological relatedness of individuals.

Mar 1, 2002 · This study revealed the nucleotide sequence of seven selected alleles at the D1S80 locus. It is suggested that DNA typing with the allelic ladder marker, which consists of all alleles analyzed in this study, can be accurately accomplished for larger …

May 1, 2006 · Students amplify DNA from their entire class, determine the size and genotype of all of the students' D1S80 loci by two gel separation techniques, compare the samples to an "evidence" sample to solve a fictitious crime, and critically evaluate the methodology.