The facility is glad to announce that we have incorporated the Cytek Aurora spectral cytometer to our core facility. It is located at the Ottawa Hospital Cancer Centre, 3rd floor, room 3.325. The instrument has 5 lasers and 64 fluorescence detection channels, allowing you to run up to 40 colors in one run

The Cytek Aurora’s use of full spectrum flow cytometry combined with the SpectroFlo ® software’s real-time unmixing capability provides greater fluorochrome choice and panel flexibility and allows users to quickly visualize data and statistics. All this results in the ultimate flow cytometry solution for deep immunoprofiling, from 24 ...

The FCV Core Facility currently houses the following instruments: BD LSRFortessa SORP, BD Celesta, Beckman Coulter MoFlo Astrios, and an AutoMACS Pro Magnetic Cell Separator (located at OHRI).

Semiconductor array for each laser enabling more efficient spectrum capture in the 420nm-830nm range. No filter changes required for any fluorochromes excited by the 405nm, 488nm and 640nm lasers. NOTE: This instrument requires additional training to use. For training, please submit the UFCR Aurora Training Request form.

The Cytek Aurora cytometer is a benchtop spectral flow cytometer. Equipped with 5 spatially separated lasers, 64 fluorescent detectors and 3 light scatter detectors for fast multicolour analysis

The Advanced Flow Cytometry Platform offers users the Cytek "Aurora" spectral flow cytometer, a next generation high end cell analyser with "full spectral" capabilities. Utilising full...

The Cytek Aurora ™ CS system combines FSP ™ technology and high-end sorting functionality to dig deep into cellular assays. Sort populations up to 64 levels deep into the gating hierarchy, up to two-way sorting with 15 mL tubes, sort up to 6 ways into 1.5 mL or 5 mL tubes, or sort into 384-well or 96-well plates with index sorting capability.

All the users of the Cytek Aurora must be trained by Fernando Ortiz or prove that they have received appropriate training (a test must be passed in other to operate the instrument). Bookings must be performed more than 24 hours in advance to facilitate usage of every researcher.

Data acquisition on Cytek Aurora. Spectral & Flow Cytometry data analysis. 1 computer at the Ottawa Hospital Cancer Centre contains the software for the usage of researchers.

The Aurora system’s implementation of full spectrum cytometry enables the use of autofluorescence extraction to further improve data clarity. Certain sample types, such as yeast and tumor samples, present the challenge of high autofluorescence.